Sergei P. Balashov

article

Electrogenic steps of light-driven proton transport in ESR, a retinal protein from Exiguobacterium sibiricum

Sergey A. Siletsky, Mahir D. Mamedov, Evgeniy P. Lukashev, Sergei P. Balashov, more

Biochimica et Biophysica Acta (BBA) - Bioenergetics > 2016 > 1857 > 11 > 1741-1750

A retinal protein from Exiguobacterium sibiricum (ESR) functions as a light-driven proton pump. Unlike other proton pumps, it contains Lys96 instead of a usual carboxylic residue in the internal proton donor site. Nevertheless, the reprotonation of the Schiff base occurs fast, indicating that Lys96 facilitates proton transfer from the bulk. In this study we examined kinetics of light-induced transmembrane...

chapter

Xanthorhodopsin

Janos K. Lanyi, Sergei P. Balashov

Halophiles and Hypersaline Environments > 319-340

Xanthorhodopsin of Salinibacter ruber is a simple system for collection of light by a carotenoid antenna, transfer of electronic excitation from the carotenoid to a retinal, and utilization of the energy gained for the active transport of protons across the membrane. As a model system, this protein, a member of the heptahelical transmembrane family of prokaryotic rhodopsins, poses numerous questions...

chapter

Participation of Internal Water Molecules and Clusters in the Unidirectional Light-Induced Proton Transfer in Bacteriorhodopsin

Akio Maeda, Sergei P. Balashov, Thomas G. Ebrey

Light Sensing in Plants > Other Photoreceptors > 213-221

Proton pumping is a fundamental biological process for generating free energy in the form of a transmembrane electrochemical potential for hydrogen ions; this energy is then utilized in cellular processes such as ATP synthesis, rotation of a flagellar motor, and ion transport across the plasma membrane. In the photosynthetic reaction center, light is used to induce charge separation and generate reducing...

article

Interaction of Antenna Carotenoid and Retinal in the Light-Driven Pumps of Salinibacter Ruber and Gloeobacter Violaceus

Janos K. Lanyi, Sergei P. Balashov, Ella S. Imasheva, Hartmut Luecke, more

Biophysical Journal > 2013 > 104 > 2 Supplement 1 > 544a

article

Computational Modeling of Excitation Energy Transfer in Xanthorhodopsin, a Model Light-Harvesting System

Eric V. Schow, Eduardo Jardón-Valadez, Espen Sagvolden, Hartmut Luecke, more

Biophysical Journal > 2012 > 102 > 3 Supplement 1 > 167a

article

Carotenoid response to retinal excitation and photoisomerization dynamics in xanthorhodopsin

Václav Šlouf, Sergei P. Balashov, Janos K. Lanyi, Tõnu Pullerits, more

Chemical Physics Letters > 2011 > 516 > 1-3 > 96-101

We present a comparative study of xanthorhodopsin, a proton pump with the carotenoid salinixanthin serving as an antenna, and the closely related bacteriorhodopsin. Upon excitation of retinal, xanthorhodopsin exhibits a wavy transient absorption pattern in the region between 470 and 540nm. We interpret this signal as due to electrochromic effect of the transient electric field of excited retinal on...

article

Removal and Reconstitution of the Carotenoid Antenna of Xanthorhodopsin

Eleonora S. Imasheva, Sergei P. Balashov, Jennifer M. Wang, Janos K. Lanyi

The Journal of Membrane Biology > 2011 > 239 > 1-2 > 95-104

Salinixanthin, a C₄₀-carotenoid acyl glycoside, serves as a light-harvesting antenna in the retinal-based proton pump xanthorhodopsin of Salinibacter ruber. In the crystallographic structure of this protein, the conjugated chain of salinixanthin is located at the protein–lipid boundary and interacts with residues of helices E and F. Its ring, with a 4-keto group, is rotated relative to the plane of...

article

Reconstitution of Gloeobacter Rhodopsin with Echinenone: Role of the 4-Keto Group

Sergei P. Balashov, Eleonora S. Imasheva, Ah Reum Choi, Kwang-Hwan Jung, more

Biochemistry > 2010 > 49 > 45 > 9792 - 9799

article

Reconstitution of Gloeobacter violaceus Rhodopsin with a Light-Harvesting Carotenoid Antenna

Eleonora S. Imasheva, Sergei P. Balashov, Ah Reum Choi, Kwang-Hwan Jung, more

Biochemistry > 2009 > 48 > 46 > 10948 - 10955

article

Efficient Approach to Determine the p K _a of the Proton Release Complex in the Photocycle of Retinal Proteins

Jia Wu, Dewang Ma, Yazhuo Wang, Ming Ming, more

The Journal of Physical Chemistry B > 2009 > 113 > 13 > 4482 - 4491

article

Femtosecond Carotenoid to Retinal Energy Transfer in Xanthorhodopsin

Tomáš Polívka, Sergei P. Balashov, Pavel Chábera, Eleonora S. Imasheva, more

Biophysical Journal > 2009 > 96 > 6 > 2268-2277

Xanthorhodopsin of the extremely halophilic bacterium Salinibacter ruber represents a novel antenna system. It consists of a carbonyl carotenoid, salinixanthin, bound to a retinal protein that serves as a light-driven transmembrane proton pump similar to bacteriorhodopsin of archaea. Here we apply the femtosecond transient absorption technique to reveal the excited-state dynamics of salinixanthin...

article

Excitation Energy-Transfer and the Relative Orientation of Retinal and Carotenoid in Xanthorhodopsin

Sergei P. Balashov, Eleonora S. Imasheva, Jennifer M. Wang, Janos K. Lanyi

Biophysical Journal > 2008 > 95 > 5 > 2402-2414

The cell membrane of Salinibacter ruber contains xanthorhodopsin, a light-driven transmembrane proton pump with two chromophores: a retinal and the carotenoid, salinixanthin. Action spectra for transport had indicated that light absorbed by either is utilized for function. If the carotenoid is an antenna in this protein, its excited state energy has to be transferred to the retinal and should be detected...

article

P/12 Interaction of antenna carotenoid and retinal in the light-driven proton pump xanthorhodopsin

Janos K. Lanyi, Sergei P. Balashov

BBA - Bioenergetics > 2008 > 1777 > Supplement > S5

article

Xanthorhodopsin: A bacteriorhodopsin-like proton pump with a carotenoid antenna

Janos K. Lanyi, Sergei P. Balashov

BBA - Bioenergetics > 2008 > 1777 > 7-8 > 684-688

Xanthorhodopsin is a light-driven proton pump like bacteriorhodopsin, but made more effective for collecting light by its second chromophore, salinixanthin, a carotenoid. Action spectra for transport and fluorescence of the retinal upon excitation of the carotenoid indicate that the carotenoid functions as an antenna to the retinal. The calculated center-to-center distance and angle of the transition...

article

Functions of carotenoids in xanthorhodopsin and archaerhodopsin, from action spectra of photoinhibition of cell respiration

Vladimir A. Boichenko, Jennifer M. Wang, Josefa Antón, Janos K. Lanyi, more

BBA - Bioenergetics > 2006 > 1757 > 12 > 1649-1656

The recent discovery of a carotenoid light-harvesting antenna in xanthorhodopsin, a retinal-based proton pump in Salinibacter ruber, made use of photoinhibition of respiration in whole cells to obtain action spectra [Balashov et al. Science 309, (2005) 2061–2064]. Here we provide further details of this phenomenon, and compare action spectra in three different systems where carotenoids have different...

article

Induced Chirality of the Light-Harvesting Carotenoid Salinixanthin and Its Interaction with the Retinal of Xanthorhodopsin ^†

Sergei P. Balashov, Eleonora S. Imasheva, Janos K. Lanyi

Biochemistry > 2006 > 45 > 36 > 10998 - 11004

article

pH Dependence of Light-Driven Proton Pumping by an Archaerhodopsin from Tibet: Comparison with Bacteriorhodopsin

Ming Ming, Miao Lu, Sergei P. Balashov, Thomas G. Ebrey, more

Biophysical Journal > 2006 > 90 > 9 > 3322-3332

The pH-dependence of photocycle of archaerhodopsin 4 (AR4) was examined, and the underlying proton pumping mechanism investigated. AR4 is a retinal-containing membrane protein isolated from a strain of halobacteria from a Tibetan salt lake. It acts as a light-driven proton pump like bacteriorhodopsin (BR). However, AR4 exhibits an “abnormal” feature—the time sequence of proton release and uptake is...

article

Formation of a Long-Lived Photoproduct with a Deprotonated Schiff Base in Proteorhodopsin, and Its Enhancement by Mutation of Asp227 ^†

Eleonora S. Imasheva, Kazumi Shimono, Sergei P. Balashov, Jennifer M. Wang, more

Biochemistry > 2005 > 44 > 32 > 10828 - 10838

article

Relocation of Water Molecules between the Schiff Base and the Thr46−Asp96 Region during Light-Driven Unidirectional Proton Transport by Bacteriorhodopsin: An FTIR Study of the N Intermediate ^†

Akio Maeda, Robert B. Gennis, Sergei P. Balashov, Thomas G. Ebrey

Biochemistry > 2005 > 44 > 16 > 5960 - 5968

article

Selectivity of Retinal Photoisomerization in Proteorhodopsin Is Controlled by Aspartic Acid 227 ^†

Eleonora S. Imasheva, Sergei P. Balashov, Jennifer M. Wang, Andrei K. Dioumaev, more

Biochemistry > 2004 > 43 > 6 > 1648 - 1655

INFONA - science communication portal

Search results for: Sergei P. Balashov

Electrogenic steps of light-driven proton transport in ESR, a retinal protein from Exiguobacterium sibiricum

Xanthorhodopsin

Participation of Internal Water Molecules and Clusters in the Unidirectional Light-Induced Proton Transfer in Bacteriorhodopsin

Interaction of Antenna Carotenoid and Retinal in the Light-Driven Pumps of Salinibacter Ruber and Gloeobacter Violaceus

Computational Modeling of Excitation Energy Transfer in Xanthorhodopsin, a Model Light-Harvesting System

Carotenoid response to retinal excitation and photoisomerization dynamics in xanthorhodopsin

Removal and Reconstitution of the Carotenoid Antenna of Xanthorhodopsin

Reconstitution of Gloeobacter Rhodopsin with Echinenone: Role of the 4-Keto Group

Reconstitution of Gloeobacter violaceus Rhodopsin with a Light-Harvesting Carotenoid Antenna

Efficient Approach to Determine the p K _a of the Proton Release Complex in the Photocycle of Retinal Proteins

Femtosecond Carotenoid to Retinal Energy Transfer in Xanthorhodopsin

Excitation Energy-Transfer and the Relative Orientation of Retinal and Carotenoid in Xanthorhodopsin

P/12 Interaction of antenna carotenoid and retinal in the light-driven proton pump xanthorhodopsin

Xanthorhodopsin: A bacteriorhodopsin-like proton pump with a carotenoid antenna

Functions of carotenoids in xanthorhodopsin and archaerhodopsin, from action spectra of photoinhibition of cell respiration

Induced Chirality of the Light-Harvesting Carotenoid Salinixanthin and Its Interaction with the Retinal of Xanthorhodopsin ^†

pH Dependence of Light-Driven Proton Pumping by an Archaerhodopsin from Tibet: Comparison with Bacteriorhodopsin

Formation of a Long-Lived Photoproduct with a Deprotonated Schiff Base in Proteorhodopsin, and Its Enhancement by Mutation of Asp227 ^†

Relocation of Water Molecules between the Schiff Base and the Thr46−Asp96 Region during Light-Driven Unidirectional Proton Transport by Bacteriorhodopsin: An FTIR Study of the N Intermediate ^†

Selectivity of Retinal Photoisomerization in Proteorhodopsin Is Controlled by Aspartic Acid 227 ^†

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