Search results for: David W. Starke

According to their demonstrated activities, the thiol-disulfide oxidoreductase (TDOR) enzyme systems [thioltransferase (glutaredoxin) and GSSG reductase; and thioredoxin and thioredoxin reductase] are expected to provide the primary cellular mechanism for protection and repair of sulfhydryl proteins under oxidative stress. Since all four enzymes have active site dithiol moieties, they may be vulnerable...

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Cloning, expression and characterization of human thioltransferase (glutaredoxin) in E. coli

Carol A. Chrestensen, Christopher B. Eckman, David W. Starke, John J. Mieyal

FEBS Letters > 1995 > 374 > 1 > 25-28

PCR primers were designed from the known amino acid (aa) sequence for human red blood cell thioltransferase (hRBC TTase) and the known cDNA sequence for pig liver TTase (82% homologous) and used to amplify thioltransferase from a pool of human brain cDNAs. The PCR product was inserted into the pKK233-2 expression vector. The DNA sequence of the insert agreed with the aa sequence. High level expression...

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Search results for: David W. Starke

Kinetic and Mechanistic Characterization and Versatile Catalytic Properties of Mammalian Glutaredoxin 2: Implications for Intracellular Roles ^†

Selective Inactivation of Glutaredoxin by Sporidesmin and Other Epidithiopiperazinediones ^† ^, ^‡

Computational and Mutational Analysis of Human Glutaredoxin (Thioltransferase): Probing the Molecular Basis of the Low p K _a of Cysteine 22 and Its Role in Catalysis ^† ^, ^‡ ^, ^§

Reversible Inactivation of α-Ketoglutarate Dehydrogenase in Response to Alterations in the Mitochondrial Glutathione Status ^†

Sensitivity of Protein Sulfhydryl Repair Enzymes to Oxidative Stress

Cloning, expression and characterization of human thioltransferase (glutaredoxin) in E. coli

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INFONA - science communication portal

Search results for: David W. Starke

Kinetic and Mechanistic Characterization and Versatile Catalytic Properties of Mammalian Glutaredoxin 2: Implications for Intracellular Roles †

Selective Inactivation of Glutaredoxin by Sporidesmin and Other Epidithiopiperazinediones † , ‡

Computational and Mutational Analysis of Human Glutaredoxin (Thioltransferase): Probing the Molecular Basis of the Low p K a of Cysteine 22 and Its Role in Catalysis † , ‡ , §

Reversible Inactivation of α-Ketoglutarate Dehydrogenase in Response to Alterations in the Mitochondrial Glutathione Status †

Sensitivity of Protein Sulfhydryl Repair Enzymes to Oxidative Stress

Cloning, expression and characterization of human thioltransferase (glutaredoxin) in E. coli

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Kinetic and Mechanistic Characterization and Versatile Catalytic Properties of Mammalian Glutaredoxin 2: Implications for Intracellular Roles ^†

Selective Inactivation of Glutaredoxin by Sporidesmin and Other Epidithiopiperazinediones ^† ^, ^‡

Computational and Mutational Analysis of Human Glutaredoxin (Thioltransferase): Probing the Molecular Basis of the Low p K _a of Cysteine 22 and Its Role in Catalysis ^† ^, ^‡ ^, ^§

Reversible Inactivation of α-Ketoglutarate Dehydrogenase in Response to Alterations in the Mitochondrial Glutathione Status ^†