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High-performance liquid chromatography with mercury cathode electrochemical detection (HPLC–EC(Hg)) was used to separate and quantify various phospholipid hydroperoxide (PLOOH) families. Under the conditions used, baseline separation of four major biologically relevant PLOOH classes was achieved. Responsiveness was linear up to at least 1nmol of PLOOH with a detection limit in the subpicomolar range...
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