Search results for: Shouming He

Items from 1 to 9 out of 9 results

article

A Mechanism-Based ICAT Strategy for Comparing Relative Expression and Activity Levels of Glycosidases in Biological Systems

Omid Hekmat, Shouming He, R. Antony J. Warren, Stephen G. Withers

Journal of Proteome Research > 2008 > 7 > 8 > 3282 - 3292

article

Identification of the catalytic nucleophile in Family 42 β-galactosidases by intermediate trapping and peptide mapping: YesZ from Bacillus subtilis

Fathima Aidha Shaikh, Johannes Müllegger, Shouming He, Stephen G. Withers

FEBS Letters > 2007 > 581 > 13 > 2441-2446

The mechanism-based inhibitor 2,4-dinitrophenyl 2-deoxy-2-fluoro-β-d-galactopyranoside (DNP2FGal) was used to inactivate the Family 42 β-galactosidase (YesZ) from Bacillus subtilis via the trapping of a glycosyl-enzyme intermediate, thereby tagging the catalytic nucleophile in the active site. Proteolytic digestion of the inactivated enzyme and of a control sample of unlabeled enzyme, followed by...

article

Family 39 α- l -Iduronidases and β- d -Xylosidases React through Similar Glycosyl−Enzyme Intermediates: Identification of the Human Iduronidase Nucleophile ^†

Catharine E. Nieman, Alexander W. Wong, Shouming He, John J. Hopwood, more

Biochemistry > 2003 > 42 > 26 > 8054 - 8065

article

Reaction of 1-Amino-2-methylenecyclopropane-1-carboxylate with 1-Aminocyclopropane-1-carboxylate Deaminase: Analysis and Mechanistic Implications ^†

Zongbao Zhao, Huawei Chen, Keqiang Li, Wensheng Du, more

Biochemistry > 2003 > 42 > 7 > 2089 - 2103

article

Radical Formation at Tyr39 and Tyr153 Following Reaction of Yeast Cytochrome c Peroxidase with Hydrogen Peroxide ^†

Haoming Zhang, Shouming He, A. Grant Mauk

Biochemistry > 2002 > 41 > 46 > 13507 - 13513

article

A Revised Mechanism for the Inactivation of Bovine Liver Enoyl-CoA Hydratase by (Methylenecyclopropyl)formyl-CoA Based on Unexpected Results with the C114A Mutant ^†

Gautam Agnihotri, Shouming He, Lin Hong, Srikanth Dakoji, more

Biochemistry > 2002 > 41 > 6 > 1843 - 1852

article

The identification of the catalytic nucleophiles of two β-galactosidases from glycoside hydrolase family 35

Jan E Blanchard, Laurent Gal, Shouming He, Janine Foisy, more

Carbohydrate Research > 2001 > 333 > 1 > 7-17

The β-galactosidases from Xanthomonas manihotis (β-Gal Xmn) and Bacillus circulans (β-Gal-3 Bcir) are retaining glycosidases that hydrolyze glycosidic bonds through a double displacement mechanism involving a covalent glycosyl–enzyme intermediate. The mechanism-based inactivator 2,4-dinitrophenyl 2-deoxy-2-fluoro-β-d-galactopyranoside was shown to inactivate β-Gal Xmn and β-Gal-3 Bcir through the...

article

The synthesis, testing and use of 5-fluoro-α-d-galactosyl fluoride to trap an intermediate on green coffee bean α-galactosidase and identify the catalytic nucleophile

Hoa D. Ly, Steven Howard, Kelly Shum, Shouming He, more

Carbohydrate Research > 2000 > 329 > 3 > 539 - 547

5-Fluoro-α-d-galactopyranosyl fluoride was synthesized and its interaction with the active site of an α-galactosidase from green coffee bean (Coffea arabica), a retaining glycosidase, characterized kinetically and structurally. The compound behaves as an apparently tight binding (K _i =600 nM) competitive inhibitor, achieving this high affinity through reaction as a slow substrate that accumulates...

article

Identification of Asp197 as the catalytic nucleophile in the family 38 α-mannosidase from bovine kidney lysosomes

Shin Numao, Shouming He, Gry Evjen, Steven Howard, more

FEBS Letters > 2000 > 484 > 3 > 175-178

Bovine kidney lysosomal α-mannosidase is a family 38 α-mannosidase involved in the degradation of glycoproteins. The mechanism-based reagent, 5-fluoro-β-L-gulosyl fluoride, was used to trap a glycosyl-enzyme intermediate, thereby labelling the catalytic nucleophile of this enzyme. After proteolytic digestion and high performance liquid chromatography/tandem mass spectrometry (MS) analysis, a labelled...

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Keywords

MASS SPECTROMETRY (3)
GLYCOSIDASE (2)
5-FLUORO-Α-D-GALACTOPYRANOSYL FLUORIDE (1)
BACILLUS SUBTILIS Β-GALACTOSIDASES (1)
CATALYTIC NUCLEOPHILE (1)
COVALENT GLYCOSYL-ENZYME INTERMEDIATE (1)
ENZYME MECHANISM (1)
FLUOROSUGARS (1)
GALACTOSIDASE (1)
GLYCOSIDASES (1)
GLYCOSIDE HYDROLASE (1)
INTERMEDIATE TRAPPING (1)
LABELING (1)
LYSOSOMAL Α-MANNOSIDASE (1)
MECHANISM-BASED INACTIVATOR (1)
NUCLEOPHILE (1)
SLOW SUBSTRATE (1)
Α-GALACTOSIDASE (1)
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ACS (5)
Elsevier (4)

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Search results for: Shouming He

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