Search results for: Emilia T. Mollova

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article

Fast high-resolution mapping of long fragments of genomic DNA based on single-molecule detection

Ekaterina Protozanova, Meng Zhang, Eric J. White, Emilia T. Mollova, more

Analytical Biochemistry > 2010 > 402 > 1 > 83-90

Here we describe bacterial genotyping by direct linear analysis (DLA) single-molecule mapping. DLA involves preparation of restriction digest of genomic DNA labeled with a sequence-specific fluorescent probe and stained nonspecifically with intercalator. These restriction fragments are stretched one by one in a microfluidic device, and the distribution of probes on the fragments is determined by single-molecule...

article

An automated sample preparation system with mini-reactor to isolate and process submegabase fragments of bacterial DNA

Emilia T. Mollova, Vishal A. Patil, Ekaterina Protozanova, Meng Zhang, more

Analytical Biochemistry > 2009 > 391 > 2 > 135-143

Existing methods for extraction and processing of large fragments of bacterial genomic DNA are manual, time-consuming, and prone to variability in DNA quality and recovery. To solve these problems, we have designed and built an automated fluidic system with a mini-reactor. Balancing flows through and tangential to the ultrafiltration membrane in the reactor, cells and then released DNA can be immobilized...

article

Probing the Dynamics of the P1 Helix within the Tetrahymena Group I Intron

Xuesong Shi, Emilia T. Mollova, Goran Pljevaljčić, David P. Millar, more

Journal of the American Chemical Society > 2009 > 131 > 27 > 9571 - 9578

article

Single-molecule fluorescence of nucleic acids

Emilia T. Mollova

Current Opinion in Chemical Biology > 2002 > 6 > 6 > 823-828

Less than a decade old, single-molecule fluorescence of nucleic acids has rapidly become an important tool in the arsenal of biological probes. A variety of novel approaches to investigate conformational dynamics, catalytic mechanisms, folding pathways and protein-nucleic-acid interactions have recently been devised for nucleic acids using this technique. Combined with biomechanical tools and ensemble...

article

The Global Conformation of the Hammerhead Ribozyme Determined Using Residual Dipolar Couplings ^†

Kent Bondensgaard, Emilia T. Mollova, Arthur Pardi

Biochemistry > 2002 > 41 > 39 > 11532 - 11542

article

NMR solution structure determination of RNAs

Emilia T Mollova, Arthur Pardi

Current Opinion in Structural Biology > 2000 > 10 > 3 > 298-302

During the past several years, there have been significant advances in NMR solution structure determination of macromolecules. The ability to easily measure residual dipolar couplings, to directly detect NH...N hydrogen bonding interactions and to study much larger macromolecules by the application of heteronuclear experiments that select narrow lines in 2D and 3D spectra of isotopically labeled molecules...

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Search results for: Emilia T. Mollova

Fast high-resolution mapping of long fragments of genomic DNA based on single-molecule detection

An automated sample preparation system with mini-reactor to isolate and process submegabase fragments of bacterial DNA

Probing the Dynamics of the P1 Helix within the Tetrahymena Group I Intron

Single-molecule fluorescence of nucleic acids

The Global Conformation of the Hammerhead Ribozyme Determined Using Residual Dipolar Couplings †

NMR solution structure determination of RNAs

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The Global Conformation of the Hammerhead Ribozyme Determined Using Residual Dipolar Couplings ^†