Search results for: Marilyn Schuman Jorns

NikD is an unusual amino-acid-oxidizing enzyme that contains covalently bound FAD, catalyzes a 4-electron oxidation of piperideine-2-carboxylic acid to picolinate, and plays a critical role in the biosynthesis of nikkomycin antibiotics. Crystal structures of closed and open forms of nikD, a two-domain enzyme, have been determined to resolutions of 1.15 and 1.9 Å, respectively. The two forms differ...

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A Mobile Tryptophan Is the Intrinsic Charge Transfer Donor in a Flavoenzyme Essential for Nikkomycin Antibiotic Biosynthesis ^†

Robert C. Bruckner, Gouhua Zhao, Patricia Ferreira, Marilyn Schuman Jorns

Biochemistry > 2007 > 46 > 3 > 819 - 827

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Identification of a Stable Flavin-thiolate Adduct in Heterotetrameric Sarcosine Oxidase

Robert M.G. Hynson, F. Scott Mathews, Marilyn Schuman Jorns

Journal of Molecular Biology > 2006 > 362 > 4 > 656-663

Heterotetrameric sarcosine oxidase (TSOX) is a complex bifunctional flavoenzyme that contains two flavins. Most of the FMN in recombinant TSOX is present as a covalent adduct with an endogenous ligand. Enzyme denaturation disrupts the adduct, accompanied by release of a stoichiometric amount of sulfide. Enzyme containing ≥90% unmodified FMN is prepared by displacement of the endogenous ligand with...

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Role of the Covalent Flavin Linkage in Monomeric Sarcosine Oxidase ^†

Alshaimaa Hassan-Abdallah, Guohua Zhao, Marilyn Schuman Jorns

Biochemistry > 2006 > 45 > 31 > 9454 - 9462

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Heterotetrameric Sarcosine Oxidase: Structure of a Diflavin Metalloenzyme at 1.85 Å Resolution

Zhi-wei Chen, Alshaimaa Hassan-Abdulah, Gouhua Zhao, Marilyn Schuman Jorns, more

Journal of Molecular Biology > 2006 > 360 > 5 > 1000-1018

The crystal structure of heterotetrameric sarcosine oxidase (TSOX) from Pseudomonas maltophilia has been determined at 1.85 Å resolution. TSOX contains three coenzymes (FAD, FMN and NAD ⁺ ), four different subunits (α, 103 kDa; β, 44 kDa; γ, 21 kDa; δ, 11 kDa) and catalyzes the oxidation of sarcosine (N-methylglycine) to yield hydrogen peroxide, glycine and formaldehyde. In the presence of...

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Spectral and Kinetic Characterization of the Michaelis Charge Transfer Complex in Monomeric Sarcosine Oxidase ^†

Gouhua Zhao, Marilyn Schuman Jorns

Biochemistry > 2006 > 45 > 19 > 5985 - 5992

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Ionization of Zwitterionic Amine Substrates Bound to Monomeric Sarcosine Oxidase ^†

Gouhua Zhao, Marilyn Schuman Jorns

Biochemistry > 2005 > 44 > 51 > 16866 - 16874

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Structure of the Sodium Borohydride-Reduced N -(Cyclopropyl)glycine Adduct of the Flavoenzyme Monomeric Sarcosine Oxidase ^† ^, ^‡

Zhi-wei Chen, Gouhua Zhao, Suzana Martinovic, Marilyn Schuman Jorns, more

Biochemistry > 2005 > 44 > 47 > 15444 - 15450

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Cloning, expression and crystallization of heterotetrameric sarcosine oxidase from Pseudomonas maltophilia

Alshaimaa Hassan-Abdallah, Guohua Zhao, Michael Eschenbrenner, Zhi-wei Chen, more

Protein Expression and Purification > 2005 > 43 > 1 > 33-43

Heterotetrameric sarcosine oxidase (TSOX) is a complex bifunctional enzyme that catalyzes the oxidation of the methyl group in sarcosine (N-methylglycine) and transfer of the oxidized methyl group into the one-carbon metabolic pool. In addition to four different subunits, TSOX contains three coenzymes (FAD, FMN, and NAD) and a binding site for tetrahydrofolate, the coenzyme acceptor of the oxidized...

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Biosynthesis of Covalently Bound Flavin: Isolation and in Vitro Flavinylation of the Monomeric Sarcosine Oxidase Apoprotein ^†

Alshaimaa Hassan-Abdallah, Robert C. Bruckner, Guohua Zhao, Marilyn Schuman Jorns

Biochemistry > 2005 > 44 > 17 > 6452 - 6462

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Nikkomycin Biosynthesis: Formation of a 4-Electron Oxidation Product during Turnover of NikD with Its Physiological Substrate ^†

Robert C. Bruckner, Guohua Zhao, David Venci, Marilyn Schuman Jorns

Biochemistry > 2004 > 43 > 28 > 9160 - 9167

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Tautomeric Rearrangement of a Dihydroflavin Bound to Monomeric Sarcosine Oxidase or N -Methyltryptophan Oxidase ^†

Peeyush Khanna, Marilyn Schuman Jorns

Biochemistry > 2003 > 42 > 4 > 864 - 869

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Search results for: Marilyn Schuman Jorns

Probing Oxygen Activation Sites in Two Flavoprotein Oxidases Using Chloride as an Oxygen Surrogate

Pleiotropic Impact of a Single Lysine Mutation on Biosynthesis of and Catalysis by N -Methyltryptophan Oxidase

Structural Characterization of Mutations at the Oxygen Activation Site in Monomeric Sarcosine Oxidase,

Factors That Affect Oxygen Activation and Coupling of the Two Redox Cycles in the Aromatization Reaction Catalyzed by NikD, an Unusual Amino Acid Oxidase

Probing the Role of Active Site Residues in NikD, an Unusual Amino Acid Oxidase That Catalyzes an Aromatization Reaction Important in Nikkomycin Biosynthesis

Spectral and Kinetic Characterization of Intermediates in the Aromatization Reaction Catalyzed by NikD, an Unusual Amino Acid Oxidase

Identification of the Oxygen Activation Site in Monomeric Sarcosine Oxidase: Role of Lys265 in Catalysis ^†

Covalent Flavinylation of Monomeric Sarcosine Oxidase: Identification of a Residue Essential for Holoenzyme Biosynthesis ^†

NikD, an Unusual Amino Acid Oxidase Essential for Nikkomycin Biosynthesis: Structures of Closed and Open Forms at 1.15 and 1.90 Å Resolution

A Mobile Tryptophan Is the Intrinsic Charge Transfer Donor in a Flavoenzyme Essential for Nikkomycin Antibiotic Biosynthesis ^†

Identification of a Stable Flavin-thiolate Adduct in Heterotetrameric Sarcosine Oxidase

Role of the Covalent Flavin Linkage in Monomeric Sarcosine Oxidase ^†

Heterotetrameric Sarcosine Oxidase: Structure of a Diflavin Metalloenzyme at 1.85 Å Resolution

Spectral and Kinetic Characterization of the Michaelis Charge Transfer Complex in Monomeric Sarcosine Oxidase ^†

Ionization of Zwitterionic Amine Substrates Bound to Monomeric Sarcosine Oxidase ^†

Structure of the Sodium Borohydride-Reduced N -(Cyclopropyl)glycine Adduct of the Flavoenzyme Monomeric Sarcosine Oxidase ^† ^, ^‡

Cloning, expression and crystallization of heterotetrameric sarcosine oxidase from Pseudomonas maltophilia

Biosynthesis of Covalently Bound Flavin: Isolation and in Vitro Flavinylation of the Monomeric Sarcosine Oxidase Apoprotein ^†

Nikkomycin Biosynthesis: Formation of a 4-Electron Oxidation Product during Turnover of NikD with Its Physiological Substrate ^†

Tautomeric Rearrangement of a Dihydroflavin Bound to Monomeric Sarcosine Oxidase or N -Methyltryptophan Oxidase ^†

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