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Treatment of reticulocyte polysomes with elongation factor cEF-2 and GTP led to an increased sensitivity or peptidyl-tRNA for puromycin as a result of the translocation from the ribosomal A-site to the P-site, upon addition of an excess of the non-hydrolysable GTP analogue, GuoPP(CH 2 ]P, the puromycin sensitivity decreased rapidly, The decrease in sensitivity required high concentrations...
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