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We have investigated possible interaction sites for mRNA, tRNA, translation factors and the nascent peptide on 5S, 5.8S and 28S rRNA in in vivo assembled translational active mouse ribosomes by comparing the chemical footprinting patterns derived from native polysomes, salt-washed polysomes (mainly lacking translational factors) and salt-washed runoff ribosomes (lacking mRNA, tRNA and translational...
Treatment of reticulocyte polysomes with elongation factor cEF-2 and GTP led to an increased sensitivity or peptidyl-tRNA for puromycin as a result of the translocation from the ribosomal A-site to the P-site, upon addition of an excess of the non-hydrolysable GTP analogue, GuoPP(CH 2 ]P, the puromycin sensitivity decreased rapidly, The decrease in sensitivity required high concentrations...
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