Search results for: Shui-Ping So

Items from 1 to 17 out of 17 results

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Prostaglandin E2 produced by inducible COX-2 and mPGES-1 promoting cancer cell proliferation in vitro and in vivo

Diana Ruan, Shui-Ping So

Life Sciences > 2014 > 116 > 1 > 43-50

Many cancers originate and flourish in a prolonged inflammatory environment. Our aim is to understand the mechanisms of how the pathway of prostaglandin E ₂ (PGE ₂ ) biosynthesis and signaling can promote cancer growth in inflammatory environment at cellular and animal model levels.In this study, a chronic inflammation pathway was mimicked with a stable cell line that over-expressed...

article

Resistant to thrombosis, induced stroke and heart arrest by incorporation of a single gene of PGI2-synthesizing COX-1-PGIS in vivo: Implication against human heart disease

Ke-He Ruan, Anita J. Mohite, Shui-Ping So

International Journal of Cardiology > 2013 > 168 > 3 > 2960-2961

article

Establishing novel prostacyclin-synthesizing cells with therapeutic potential against heart diseases

Ke-He Ruan, Anita Mohite, Shui-Ping So, Cheng-Huai Ruan

International Journal of Cardiology > 2013 > 163 > 2 > 163-169

For decades, there have been many ongoing attempts to use prostaglandin I ₂ (PGI ₂ ) to treat heart diseases, such as pulmonary arterial hypertension. However, the short half life of PGI ₂ has limited the therapeutic impact potential.Here, we have engineered a novel adipose tissue-derived cell that constantly produces PGI _2, through transfecting of an engineered cDNA...

article

Engineered endothelial progenitor cells that overexpress prostacyclin protect vascular cells

Qi Liu, Yutao Xi, Toya Terry, Shui‐Ping So, more

Journal of Cellular Physiology > 227 > 7 > 2907 - 2916

Prostacyclin (PGI2) is a potent vasodilator and important mediator of vascular homeostasis; however, its clinical use is limited because of its short (<2‐min) half‐life. Thus, we hypothesize that the use of engineered endothelial progenitor cells (EPCs) that constitutively secrete high levels of PGI2 may overcome this limitation of PGI2 therapy. A cDNA encoding COX‐1‐10aa‐PGIS, which links human...

article

A profile of NSAID-targeted arachidonic acid metabolisms in human embryonic stem cells (hESCs): Implication of the negative effects of NSAIDs on heart tissue regeneration

Annirudha Chillar, Shui-Ping So, Cheng-Huai Ruan, Harnath Shelat, more

International Journal of Cardiology > 2011 > 150 > 3 > 253-259

An emerging technology using human embryonic stem cells (hESCs) to regenerate infarcted heart tissue has been underdeveloped. However, because non-steroidal anti-inflammatory drugs (NSAIDs), such as aspirin, are taken during the infarction, it becomes critical to know whether the NSAIDs have negative impacts on heart tissue regeneration when using hESCs.Mass spectrometry (LC/MS/MS) and high performance...

article

Novel Mechanism of the Vascular Protector Prostacyclin: Regulating MicroRNA Expression

Anita Mohite, Annirudha Chillar, Shui-Ping So, Vanessa Cervantes, more

Biochemistry > 2011 > 50 > 10 > 1691 - 1699

article

Inducible COX-2 dominates over COX-1 in prostacyclin biosynthesis: Mechanisms of COX-2 inhibitor risk to heart disease

Cheng-Huai Ruan, Shui-Ping So, Ke-He Ruan

Life Sciences > 2011 > 88 > 1-2 > 24-30

Our aim is to understand the molecular mechanisms of the selective nonsteroidal anti-inflammatory drugs (NSAID), cyclooxygenase-2 (COX-2) inhibitors', higher “priority” to reduce synthesis of the vascular protector, prostacyclin (PGI2), compared to that of nonselective NSAIDs.COX-1 or COX-2 was co-expressed with PGI2 synthase (PGIS) in COS-7 cells. The Km and initial velocity (½t Vmax) of the coupling...

article

Large-scale expression, purification, and characterization of an engineered prostacyclin-synthesizing enzyme with therapeutic potential

Ke-He Ruan, Shui-Ping So, Hanjing Wu, Vanessa Cervantes

Archives of Biochemistry and Biophysics > 2008 > 480 > 1 > 41-50

Recently, we reported that a novel hybrid enzyme (TriCat enzyme), engineered by linking human cyclooxygenase-2 (COX-2) with prostacyclin (PGI ₂ ) synthase (PGIS) together through a transmembrane domain, was able to directly integrate the triple catalytic (TripCat) functions of COX-2 and PGIS and effectively convert arachidonic acid (AA) into the vascular protector, PGI ₂ [K.H. Ruan,...

article

Involvement of non-conserved residues important for PGE2 binding to the constrained EP3 eLP2 using NMR and site-directed mutagenesis

Annirudha Chillar, Jiaxin Wu, Shui-Ping So, Ke-He Ruan

FEBS Letters > 2008 > 582 > 19 > 2863-2868

A peptide constrained to a conformation of second extracellular loop of human prostaglandin-E ₂ (PGE ₂ ) receptor subtype3 (hEP3) was synthesized. The contacts between the peptide residues at S211 and R214, and PGE ₂ were first identified by NMR spectroscopy. The results were used as a guide for site-directed mutagenesis of the hEP3 protein. The S211L and R214L mutants expressed...

article

Engineering of a Protein with Cyclooxygenase and Prostacyclin Synthase Activities That Converts Arachidonic Acid to Prostacyclin ^†

Ke-He Ruan, Hui Deng, Shui-Ping So

Biochemistry > 2006 > 45 > 47 > 14003 - 14011

article

The N-terminal membrane anchor domain of the membrane-bound prostacyclin synthase involved in the substrate presentation of the coupling reaction with cyclooxygenase

Ke-He Ruan, Hui Deng, Jiaxin Wu, Shui-Ping So

Archives of Biochemistry and Biophysics > 2005 > 435 > 2 > 372-381

To mimic the native conditions, the cyclooxygenase (COX)/prostaglandin I ₂ synthase (PGIS) coupling reaction system was used to determine the coordination of PGIS with COX for the biosynthesis of prostacyclin (PGI ₂ ) using arachidonic acid (AA) as a substrate in a membrane-bound environment. The membrane-bound PGIS exhibited a faster isomerization of PGH ₂ produced by COX...

article

Structural and functional characterization of the first intracellular loop of human thromboxane A ₂ receptor

Linda Geng, Jiaxin Wu, Shui-Ping So, Gangxiong Huang, more

Archives of Biochemistry and Biophysics > 2004 > 423 > 2 > 253-265

The conformation of a constrained peptide mimicking the putative first intracellular domain (iLP1) of thromboxane A ₂ receptor (TP) was determined by ¹ H 2D NMR spectroscopy. Through completed assignments of TOCSY, DQF-COSY, and NOESY spectra, a NMR structure of the peptide showed a β-turn in residues 56-59 and a short helical structure in the residues 63-66. It suggests that residues...

article

Evidence of the residues involved in ligand recognition in the second extracellular loop of the prostacyclin receptor characterized by high resolution 2D NMR techniques

Ke-He Ruan, Jiaxin Wu, Shui-Ping So, Lori A. Jenkins

Archives of Biochemistry and Biophysics > 2003 > 418 > 1 > 25-33

In previous studies, we have determined the solution structure of the second extracellular loop (eLP ₂ ) of the human thromboxane A ₂ receptor (TP) and identified the residues in the eLP ₂ domain involved in ligand recognition, by using a combination of approaches including a constrained synthetic peptide, 2D NMR spectroscopy, and recombinant proteins. These findings led us...

article

Solution structure of the third extracellular loop of human thromboxane A ₂ receptor

Jiaxin Wu, Shui-Ping So, Ke-He Ruan

Archives of Biochemistry and Biophysics > 2003 > 414 > 2 > 287-293

The extracellular domains of the thromboxane A ₂ receptor (TP receptor) were found to be involved in the specific ligand recognition. Determination of the three-dimensional (3D) structure of the extracellular loops would help to explain the mechanism of the ligand binding to its receptor with regard to the tertiary structure. Based on our previous studies on the extracellular loop of the human...

article

Identification of the Residues in the Helix F/G Loop Important to Catalytic Function of Membrane-Bound Prostacyclin Synthase ^†

Hui Deng, Jiaxin Wu, Shui-Ping So, Ke-He Ruan

Biochemistry > 2003 > 42 > 19 > 5609 - 5617

article

Determination of the membrane contact residues and solution structure of the helix F/G loop of prostaglandin I ₂ synthase

Jiaxin Wu, Shui-Ping So, Ke-He Ruan

Archives of Biochemistry and Biophysics > 2003 > 411 > 1 > 27-35

From our topological arrangement model of prostaglandin I ₂ synthase (PGIS) created by homology modeling and topology studies, we hypothesized that the helix F/G loop of PGIS contains a membrane contact region distinct from the N-terminal membrane anchor domain. To provide direct experimental data we have explored the relationship between the endoplasmic reticulum (ER) membrane and the PGIS...

article

Solution Structure of the Second Extracellular Loop of Human Thromboxane A ₂ Receptor ^†

Ke-He Ruan, Shui-Ping So, Jiaxin Wu, Dawei Li, more

Biochemistry > 2001 > 40 > 1 > 275 - 280

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Keywords

CYCLOOXYGENASE (5)
PROSTACYCLIN (4)
NMR (3)
SYNTHETIC PEPTIDE (3)
THROMBOXANE A 2 RECEPTOR (2)
COX-2 INHIBITOR (2)
CYTOCHROME P450 (2)
PROSTACYCLIN SYNTHASE (2)
PROSTAGLANDIN (2)
PROSTAGLANDIN I2 (2)
THROMBOXANE A 2 (1)
ANTI-INFLAMMATION (1)
ANTI-THROMBOSIS (1)
ARACHIDONIC ACID (1)
ARACHIDONIC ACID (AA) (1)
CANCERS (1)
CARDIAC ARREST (1)
COX-2 (1)
CYCLOOXYGENASE-1 (COX-1) (1)
EP3 RECEPTOR (1)
EXTRACELLULAR LOOP 2 (1)
G PROTEIN-COUPLED RECEPTOR (1)
HUMAN EMBRYONIC STEM CELLS (1)
INFLAMMATION (1)
MICROSOMAL PGE2 SYNTHASE (1)
NSAIDS (1)
PGE2 RECEPTOR (1)
PGI2 SYNTHASE (PGIS) AND FRET (1)
PROSTACYCLIN OR PROSTAGLANDIN I2 (PGI2) (1)
PROSTAGLANDIN E2 (1)
PROSTAGLANDIN E2 (PGE2) (1)
PROSTAGLANDIN I2 SYNTHASE (1)
PROSTANOID RECEPTORS (1)
PROSTANOID SYNTHASE (1)
PROTEIN ENGINEERING (1)
STROKE (1)
TRANSGENIC MICE (1)
VASCULAR PROTECTOR (1)
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Elsevier (12)
ACS (4)
Wiley (1)

Journal

Archives of Biochemistry and Biophysics (6)
Biochemistry (4)
International Journal of Cardiology (3)
Life Sciences (2)
FEBS Letters (1)
Journal of Cellular Physiology (1)

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Search results for: Shui-Ping So

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