Search results for: James L. Thomas

Items from 1 to 20 out of 84 results

article

A multichannel system integrating molecularly imprinted conductive polymers for ultrasensitive voltammetric determination of four steroid hormones in urine

Mei-Hwa Lee, James L. Thomas, Wei-Chiun Liu, Zheng-Xiang Zhang, more

Microchimica Acta > 2019 > 186 > 11 > 1-11

This work reports on a modularized electrochemical method for the determination of the hormones cortisol, progesterone, testosterone and 17β-estradiol in urine. These hormones were employed as templates when generating molecular imprints from aniline and metanilic acid by electropolymerization on the surface of screen-printed electrodes. The electrically conductive imprint was characterized by SEM,...

article

Epitope recognition of peptide-imprinted polymers for Regenerating protein 1 (REG1)

Mei-Hwa Lee, James L. Thomas, Chun-Lin Liao, Stipo Jurcevic, more

Separation and Purification Technology > 2018 > 192 > C > 213-219

Molecularly imprinted polymers (MIPs) were developed to replace natural antibodies with a cost-effective and durable synthetic material. Molecular imprinting of proteins conventionally utilizes the whole protein as the template, which is complex (as many different epitopes may be imprinted) and expensive. In this work, seven peptides (13–18 amino acids) were synthesized and used as templates for the...

article

Structure-function relationships for the selective inhibition of human 3β-hydroxysteroid dehydrogenase type 1 by a novel androgen analog

Jenny H. Pham, Catherine M. Will, Vance L. Mack, Matthew Halbert, more

The Journal of Steroid Biochemistry and Molecular Biology > 2017 > 174 > C > 257-264

3β-Hydroxysteroid dehydrogenase type 1 (3β-HSD1) is selectively expressed in human placenta, mammary glands and breast tumors in women. Human 3β-HSD2 is selectively expressed in adrenal glands and ovaries. Based on AutoDock 3 and 4 results, we have exploited key differences in the amino acid sequences of 3β-HSD1 (Ser194, Arg195) and 3β-HSD2 (Gly194, Pro195) by designing a selective inhibitor of 3β-HSD1...

article

Polymers imprinted with three REG1B peptides for electrochemical determination of Regenerating Protein 1B, a urinary biomarker for pancreatic ductal adenocarcinoma

Mei-Hwa Lee, James L. Thomas, Chun-Lin Liao, Stipo Jurcevic, more

Microchimica Acta > 2017 > 184 > 6 > 1773-1780

Three peptides (each containing 13–18 amino acids) were synthesized and used as templates for molecular imprinting and epitope recognition of the Regenerating Protein 1B (REG1B), which is one of the urinary biomarkers for pancreatic ductal adenocarcinoma (PDAC). Poly(ethylene-co-vinyl alcohol)s were employed as the host for molecular imprinting of the peptides. Following their preparation, the molecularly...

article

Electrochemical sensing of nuclear matrix protein 22 in urine with molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide nanorod arrays for clinical studies of bladder cancer diagnosis

Mei-Hwa Lee, James L. Thomas, Yu-Chia Chang, Yuh-Shyan Tsai, more

Biosensors and Bioelectronics > 2016 > 79 > C > 789-795

In 1996 and 2000, the US Food and Drug Administration (FDA) approved the use of Nuclear matrix protein 22 (NMP22) as a monitoring tool for predicting the recurrence/clearing of bladder cancer, and for screening undiagnosed individuals who have symptoms of, or are at risk for, that disease. The fabrication of electrodes for sensing NMP22 and their integration with a portable potentiostat in a homecare...

chapter

An implicit flux-split algorithm for the compressible Euler and Navier-Stokes equations

Robert W. Walters, James L. Thomas, Bram Leer

Lecture Notes in Physics > Tenth International Conference on Numerical Methods in Fluid Dynamics > 628-635

article

Recognition of Rhodobacter sphaeroides by microcontact-imprinted poly(ethylene-co-vinyl alcohol)

Mei-Hwa Lee, James L. Thomas, Ming-Huan Li, Ching-Ping Shih, more

Colloids and Surfaces B: Biointerfaces > 2015 > 135 > Complete > 394-399

The immobilization of cells or microorganisms is important for bioseparations, in bioreactors producing cellular metabolites, and as receptors for biosensing. Cell-imprinted polymers (CIPs) have been shown to have cavities with complementary shapes and also high affinities for the template cells or microorganisms. However, the effects of binding to CIPs on gene expression are only beginning to be...

article

Regulation of human 3-beta-hydroxysteroid dehydrogenase type-2 (3βHSD2) by molecular chaperones and the mitochondrial environment affects steroidogenesis

James L. Thomas, Himangshu S. Bose

Journal of Steroid Biochemistry and Molecular Biology > 2015 > 151 > Complete > 74-84

Human 3-β-hydroxysteroid dehydrogenase/isomerase types 1 and 2 (3βHSD1 and 3βHSD2, respectively) are expressed in a tissue-specific pattern by different genes. Site-directed mutagenesis studies have confirmed the function of the catalytic amino acids (Tyr154, Lys 158, Ser124 in both isoenzymes), substrate/inhibitor isoform-specific residues (His156 and Arg195 in 3βHSD1) and cofactor binding residues...

chapter

A Novel Supercritical CO₂ Synthesis of Amorphous Hydrous Zirconia Nanoparticles, and Their Calcination to Zirconia

Mei‐Hwa Lee, Hung‐Yin Lin, James L. Thomas

Progress in Nanotechnology > 129 - 135

A novel synthesis of amorphous hydrous zirconia nanoparticles was performed in a supercritical carbon dioxide reverse microemulsion, converting a high concentration of a very inexpensive starting material into a product that was then calcined to yield monoclinic zirconia nanoparticles. The amorphous hydrous zirconia precursor particles were obtained by simply adding a precipitating agent to [Zrˆ)|/perfluoropolyether/scC0...

article

Optical sensing of urinary melatonin with molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide nanorod arrays

Mei-Hwa Lee, James L. Thomas, Yi-Li Chen, Chien-Fu Lin, more

Biosensors and Bioelectronics > 2013 > 47 > Complete > 56-61

In this work, aligned zinc oxide (ZnO) nanorods were selectively hydrothermally grown on acetate-seeded spots on a gold substrate; the nanorods had an average length and diameter of 1.7μm and 240nm, respectively. Melatonin was imprinted into poly(ethylene-co-vinyl alcohol), EVAL, which was coated onto ZnO nanorod arrays. The ZnO nanorods not only increased the surface area for sensing target molecules,...

article

Synthesis of Zirconia with Nanoporous Structure by a Supercritical Carbon Dioxide Microemulsion Route

Mei‐Hwa Lee, Hung‐Yin Lin, James L. Thomas

International Journal of Applied Ceramic Technology > 7 > 6 > 874 - 880

Supercritical carbon dioxide (scCO₂) fluid as a green solvent for processing can markedly reduce the use of organic solvents. Nanoporous clusters of zirconia (ZrO₂) were synthesized in a [Zr⁴⁺](aq)/scCO₂ microemulsion using hydrazine solution as the precipitating agent, and the resulting nanostructures (surface area, morphology) under different reaction temperatures and pressures were determined by...

article

Rapid Shrinkage of Lipid-Coated Bubbles in Pulsed Ultrasound

Debra J. Cox, James L. Thomas

Ultrasound in Medicine & Biology > 2013 > 39 > 3 > 466-474

Many researchers have observed rapid shrinkage of lipid-coated microbubbles subjected to brief, MHz ultrasound pulses. The shrinkage is sometimes, but not always, accompanied by the shedding of visible fragments of the coat. It has been suggested that the shedding of the lipid coat alone is sufficient to explain the rapid shrinkage, as that loss increases bubble surface tension and, thus, internal...

article

The complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays

Mei-Hwa Lee, James L. Thomas, Yun-Chao Chen, Wei-Ti Chin, more

Microchimica Acta > 2013 > 180 > 15-16 > 1393-1399

The replacement of antibodies by molecularly imprinted polymers (MIPs) has been investigated for many decades. However, indirect protocols (including natural primary and secondary antibodies) are still utilized to evaluate the ability of MIP thin films to recognize target molecules. MIPs can be prepared as either a thin film or as particles, and cavities that are complementary to the template can...

article

Radiographic Analysis of the Canale View for Displaced Talar Neck Fractures

James L. Thomas, Brandon M. Boyce

The Journal of Foot and Ankle Surgery > 2012 > 51 > 2 > 187-190

Fractures of the talar neck comprise almost 50% of fractures of the talus and may result in significant long-term morbidity. It is of paramount importance to ensure anatomic reduction of the fracture not only for fracture healing but also for minimizing future posttraumatic arthritic sequelae. In addition to conventional radiographs and computed tomography scans, the Canale view has proven to be beneficial,...

article

Single-particle tracking of immunoglobulin E receptors (FcεRI) in micron-sized clusters and receptor patches

Kathrin Spendier, Keith A. Lidke, Diane S. Lidke, James L. Thomas

FEBS Letters > 2012 > 586 > 4 > 416-421

When mast cells contact a monovalent antigen-bearing fluid lipid bilayer, IgE-loaded FcεRI receptors aggregate at contact points and trigger degranulation and the release of immune activators. We used two-color total internal reflection fluorescence microscopy and single-particle tracking to show that most fluorescently labeled receptor complexes diffuse freely within these micron-size clusters, with...

article

Ultrasound-Induced Nanofragmentation of Bubbles with Saturated and Unsaturated Lipid Coats

Debra J. Cox, James L. Thomas

Biophysical Journal > 2012 > 102 > 3 Supplement 1 > 648a

article

Lipid-Mediated Unfolding of 3β-Hydroxysteroid Dehydrogenase 2 Is Essential for Steroidogenic Activity

Maheshinie Rajapaksha, James L. Thomas, Michael Streeter, Manoj Prasad, more

Biochemistry > 2011 > 50 > 51 > 11015 - 11024

article

Three-wire Fixation Technique for Displaced Fifth Metatarsal Base Fractures

James L. Thomas, Barry C. Davis

The Journal of Foot and Ankle Surgery > 2011 > 50 > 6 > 776-779

Fractures of the tuberosity of the fifth metatarsal are the most common type of fifth metatarsal fractures. This particular fracture usually produces low morbidity and low rates of nonunion when treated nonoperatively. However, on occasion, significant displacement, comminution, or significant intra-articular involvement may warrant operative intervention. Multiple techniques have been described for...

article

Treatment of Jones Fracture Nonunion with Isolated Intramedullary Screw Fixation

James L. Thomas, Barry C. Davis

The Journal of Foot and Ankle Surgery > 2011 > 50 > 5 > 566-568

Although the treatment of acute Jones fractures is well described in published studies, the Jones fracture nonunion is more controversial with regard to treatment. Although nonoperative treatment is an option, surgery is the usual course. We conducted a retrospective case series of 7 patients (1 man and 6 women; age range 39 to 54 years), who were initially treated nonoperatively for acute Jones fractures...

article

Sensing of Digestive Proteins in Saliva with a Molecularly Imprinted Poly(ethylene- co -vinyl alcohol) Thin Film Coated Quartz Crystal Microbalance Sensor

Mei-Hwa Lee, James L. Thomas, Hong-Yi Tseng, Wei-Che Lin, more

ACS Applied Materials & Interfaces > 2011 > 3 > 8 > 3064 - 3071

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Search results for: James L. Thomas

A multichannel system integrating molecularly imprinted conductive polymers for ultrasensitive voltammetric determination of four steroid hormones in urine

Epitope recognition of peptide-imprinted polymers for Regenerating protein 1 (REG1)

Structure-function relationships for the selective inhibition of human 3β-hydroxysteroid dehydrogenase type 1 by a novel androgen analog

Polymers imprinted with three REG1B peptides for electrochemical determination of Regenerating Protein 1B, a urinary biomarker for pancreatic ductal adenocarcinoma

Electrochemical sensing of nuclear matrix protein 22 in urine with molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide nanorod arrays for clinical studies of bladder cancer diagnosis

An implicit flux-split algorithm for the compressible Euler and Navier-Stokes equations

Recognition of Rhodobacter sphaeroides by microcontact-imprinted poly(ethylene-co-vinyl alcohol)

Regulation of human 3-beta-hydroxysteroid dehydrogenase type-2 (3βHSD2) by molecular chaperones and the mitochondrial environment affects steroidogenesis

A Novel Supercritical CO₂ Synthesis of Amorphous Hydrous Zirconia Nanoparticles, and Their Calcination to Zirconia

Optical sensing of urinary melatonin with molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide nanorod arrays

Synthesis of Zirconia with Nanoporous Structure by a Supercritical Carbon Dioxide Microemulsion Route

Rapid Shrinkage of Lipid-Coated Bubbles in Pulsed Ultrasound

The complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays

Radiographic Analysis of the Canale View for Displaced Talar Neck Fractures

Single-particle tracking of immunoglobulin E receptors (FcεRI) in micron-sized clusters and receptor patches

Ultrasound-Induced Nanofragmentation of Bubbles with Saturated and Unsaturated Lipid Coats

Lipid-Mediated Unfolding of 3β-Hydroxysteroid Dehydrogenase 2 Is Essential for Steroidogenic Activity

Three-wire Fixation Technique for Displaced Fifth Metatarsal Base Fractures

Treatment of Jones Fracture Nonunion with Isolated Intramedullary Screw Fixation

Sensing of Digestive Proteins in Saliva with a Molecularly Imprinted Poly(ethylene- co -vinyl alcohol) Thin Film Coated Quartz Crystal Microbalance Sensor

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