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Among exopolysaccharides, gellan gum is used widely as a gelling agent and food additive, and its commercial market is rapidly increasing. To optimize the fermentation production of gellan gum in Sphingomonas paucimobilis ZJU3108, carbon/nitrogen (C/N) ratio, dissolved oxygen (DO) and impeller type were studied. The C/N ratio, rather than the absolute concentration of carbon or nitrogen source, was...
Fumaric acid is an important four-carbon dicarboxylic acid as a potential biorefinery target. A high-throughput screening method for fumaric acid overproduction strains was established. Nystatin (50 mg/L) was added into the production medium to restrict the spread of Rhizopus oryzae hyphae on agar plates. With bromocerol green as a pH indicator in the agar plates, the capability of fumaric acid biosynthesis...
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