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We describe the preparation and characterization of a glassy carbon electrode modified with a bionanocomposite consisting of a hyaluronic acid, dispersed carbon nanotubes, and electrostatically bound toluidine blue. The electrode was used to detect NADH in the batch and flow-injection mode of operation. The electrode was further modified by immobilizing sorbitol dehydrogenase to result in biosensor...
A ferricyanide mediated amperometric biosensor system implementing d-sorbitol dehydrogenase together with diaphorase for sensitive detection of d-sorbitol was used. The biosensor system was successfully integrated into an off-line FIA system with a throughput of detection of 10h −1 . The device exhibited limit of detection of 20μM with an average relative standard deviation of analysis of...
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