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The paper presents two competitive enzyme linked immunosorbent assays (ELISAs) for the detection of lupine proteins in foods. The ELISAs are based on anti-lupine antibodies produced in a rabbit (IgG) and in a chicken (IgY), respectively. Cross-reactivity tests with 33 foods of plant origin revealed that both ELISAs show slight cross-reactivity (≤1.5 %) with pecan nut. They do, however, not cross-react...
The paper presents a sandwich enzyme linked immunosorbent assay (ELISA) for the detection of traces of lupine proteins in foods. Anti-lupine antibodies were produced by immunising a rabbit and a hen with a protein extract from white lupine flour. IgY were used as coating and IgG as secondary antibodies. The ELISA detects proteins from white (Lupinus albus) and blue (Lupinus angustifolius) lupine and,...
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