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The effects of cholesterol on cell proliferation and adipocytic differentiation have been evaluated for the first time in mouse adipose-derived stromal cells (mASCs). Cholesterol loading by using cholesterol:methyl- β-cyclodextrin (Chol:MβCD) complexes promoted cellular levels of free cholesterol (FC) and cholesteryl ester (CE), induced high cell proliferation of mASCs dose-dependently. Compared with...
Effects of cholesterol on osteoblastic differentiation was evaluated in mouse bone marrow derived mesenchymal stem cells (MSCs). Cholesterol-treated MSCs showed a stimulated differentiation process with induced mRNA and protein levels of osteogenic lineage markers, increased alkaline phosphatase (AKP) activity and more mineralized nodules. However, the stimulation extent was reduced when incubating...
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