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A method was developed to rapidly separate lipid classes commonly found in microorganisms. The method is based on the use of aminopropyl solid phase extraction columns to separate polyhydroxyalkanoates (PHA), phospholipids, sterols, triglycerides, diglycerides, monoglycerides, and steryl esters. Recoveries of all lipid classes, with the exception of PHA and sterols, ranged from 91% to greater than...
2,4-Dichlorophenol hydroxylase, an enzyme involved in the bacterial degradation of the herbicide 2,4-dichlorophenoxyacetate (2,4-D) was purified from two bacterial strains that harbored the same 2,4-D plasmid, pJP4. The purified enzymes (M r 224000) from the two transconjugants were indistinguishable; they contained FAD and were composed of non-identical subunits, M r 67000 and 45000,...
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