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The goal of this study was to determine the effects of various compounds on the 17-β-estradiol-induced dimerization of the human estrogen receptor alpha (hERα), a nuclear transcription factor. For this purpose, we used a modified yeast two-hybrid (YTH) bioassay designed to study protein–protein interactions, based on the electrochemical monitoring of hERα dimerization and detected as β-d-galactosidase...
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