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Mycobacterial carbohydrate sulfotransferase Stf0 catalyzes the sulfuryl group transfer from 3′-phosphoadenosine-5′-phosphosulfate (PAPS) to trehalose. The sulfation of trehalose is required for the biosynthesis of sulfolipid-1, the most abundant sulfated metabolite found in Mycobacterium tuberculosis. In this paper, an efficient enzyme kinetics assay for Stf0 using electrospray ionization (ESI) mass...
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