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Optical detection continues to dominate detection methods in microfluidics due to its noninvasive nature, easy coupling, rapid response, and high sensitivity. In this review, we summarize two aspects of recent developments in optical detection methods on microfluidic chips. The first aspect is free-space (off-chip) detection on the microchip, in which the conventional absorption, fluorescence, chemiluminescence,...
Detection of the abnormal prion protein in blood and cerebral spinal fluid of transmissible spongiform encephalophathy (TSE) infected individuals has not been possible by Western blot, immunohistochemistry or the present ELISA tests. We used an analytical approach in conjugation with a fluorescence immunoassay to develop methods to measure the abnormal prion in blood and cerebral spinal fluid. Monoclonal...
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