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A new time-resolved fluoroimmunoassay (TR-FIA) of testosterone in serum is described, using a biotinylated testosterone tracer, with a long spacer arm between biotin and testosterone, coupled to the C3 of the testosterone: a biotinylaminodecane carboxymethyloxime testosterone.This tracer affords a great sensitivity of the standard curve, because a amount of 0.3pg of testosterone can be significantly...
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