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To examine the effect of patient and facility level factors on the success of preimplantation genetic diagnosis (PGD) in patients with neurofibromatosis 1 (NF1).Retrospective review.Large PGD reference laboratory.All patients with NF1 referred from June 2004 to May 2013.None.Embryos' NF1 mutation status and live birth rates.Seventy-seven couples underwent 156 PGD cycles during the study period. The...
To cryopreserve micromanipulated ooplast segments (microcytoplasts) from mouse oocytes, compare microcytoplast and parent or recipient oocyte fusion performed within or without the zona pellucida, compare electrofusion of fresh or frozen oocyte with frozen-thawed microcytoplasts, and assess spindle integrity after reconstruction of oocytes.Prospective experimental study.University-based experimental...
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