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The fluoroquinolones, temafloxacin, sarafloxacin, and difloxacin, are determined in the bulk drug substances and in a variety of dosage forms using high-performance liquid chromatography (HPLC). The HPLC system used is also applicable for ciprofloxacin and norfloxacin. The procedure uses UV detection at 280 nm, which provides a linear response of the subject compounds to at least 20 µg/ml. Assay precision...
Spectinomycin dihydrochloride is determined by liquid chromatography with electrochemical detection. The drug is chromatographed on a reverse-phase Nucleosil C18 column using an eluent containing 0.02 M sodium citrate and 0.0015 M octyl sodium sulfate (pH 6.10 with perchloric acid) and acetonitrile (100:4). Detection is performed using a coulometric detector (porous carbon working electrode) at +...
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