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The aim of this study was to evaluate the use of antifreeze protein type III (AFP III) into vitrification medium on meiotic spindle morphology of in vitro matured bovine oocytes as well as the fertilization and blastocyst rates. Mature cumulus-oocyte complexes (COC) were distributed in four groups: control (untreated), vitrified without supplementation (AFP0) or supplemented with 500 (AFP500) or 1000 ng/mL...
The objectives of this study were to evaluate the ovarian response of oocyte donor goats which underwent seven repeated ovulation hormonal treatments and laparoscopic ovum pick-up (Experiment 1), and to compare the In vitro production (IVP) of embryos from goats hormonally treated for eighth time to IVP of embryos from females with a single hormonal treatment (Experiment 2). In Experiment 1, 12 goats...
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