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The aim of this study was to develop nomograms for predicting prostate cancer and its zonal location using prostate‐specific antigen density, prostate volume, and their zone‐adjusted derivatives. A total of 928 consecutive patients with prostate‐specific antigen (PSA) less than 20.0 ng/mL, who underwent transrectal ultrasound‐guided transperineal 12‐core prostate biopsy at West China Hospital between...
Enrichment and propagation of gonocytes or spermatogonial stem cells (SSCs) from cryopreserved testicular tissue is essential to apply SSCs‐related techniques in large domestic animals. We previously reported the cryopreservation of adult bovine testicular tissue. Here, we conducted the enrichment and culture of putative gonocytes from cryopreserved testicular tissues of post‐natal 1‐day‐old bulls...
Many studies have addressed the hazardous role of cigarette smoking on male fertility, but the exact molecular mechanisms involved in the impairments caused by nicotine remain unclear. To evaluate the detrimental effects of nicotine exposure on spermatogenesis, two‐dimensional gel electrophoresis and mass spectrometry analysis were performed to screen and identify differentially expressed proteins...
On the basis of the unknown tags in the mature human sperm serial analysis of gene expression library constructed by our laboratory, some transcripts were cloned, including Iqcf1 (IQ motif containing F1). To investigate the function of sperm‐retained Iqcf1 in spermatogenesis and fertilization of mice, we investigated the spatial and temporal expression of IQCF1. By using the (transcription activator‐like...
Spermiogenesis is the final phase of spermatogenesis. During this process, haploid round spermatids differentiate into spermatozoa, with dramatic morphological changes, including elongation and condensation of the nuclei, and formation of the flagella. Meig1 is one of many genes involved in the regulation of this process. Male mice deficient in MEIG1 are sterile with a severe defect in spermiogenesis,...
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