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Described are methods for determining the potency of somatotropins. The level of biologically active bovine somatotropin protein in a bovine somatotropin sample is measured by size exclusion HPLC employing as the stationary phase a hydrophilic porous gel having an average particle diameter of about 5 mum to about 15 mum and the a mobile phase a buffered aqueous solution which is non-denaturing to...
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