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LipC12, a true lipase from family I.1 of bacterial lipases which was previously isolated through a metagenomics approach, contains 293 amino acids. Among lipases of known three‐dimensional structure, it has a sequence identity of 47% to the lipase from Pseudomonas aeruginosa PAO1. Recombinant N‐terminally His6‐tagged LipC12 protein was expressed in Escherichia coli, purified in a homogenous form and...
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