Search results for: Tadashi Teshima

Items from 1 to 13 out of 13 results

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Editorial

Luis Moroder, Yoshiaki Kiso, Ulf Diederichsen, Tadashi Teshima

Journal of Peptide Science > 23 > 7-8 > 471 - 471

article

Erratum to: Uptake of a fluorescent l-glucose derivative 2-NBDLG into three-dimensionally accumulating insulinoma cells in a phloretin-sensitive manner

Ayako Sasaki, Katsuhiro Nagatomo, Koki Ono, Toshihiro Yamamoto, more

Human Cell > 2016 > 29 > 3 > 138-139

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Uptake of a fluorescent l-glucose derivative 2-NBDLG into three-dimensionally accumulating insulinoma cells in a phloretin-sensitive manner

Ayako Sasaki, Katsuhiro Nagatomo, Koki Ono, Toshihiro Yamamoto, more

Human Cell > 2016 > 29 > 1 > 37-45

Of two stereoisomers of glucose, only d- and not l-glucose is abundantly found in nature, being utilized as an essential fuel by most organisms. The uptake of d-glucose into mammalian cells occurs through glucose transporters such as GLUTs, and this process has been effectively monitored by a fluorescent d-glucose derivative 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-NBDG)...

article

An analysis of glucose uptake into neurons and astrocytes using 2-NBDG combined with newly synthesized fluorescent L-glucose derivatives

Katsuya Yamada, Katsuhiro Nagatomo, Sechiko Suga, Toshihiro Yamamoto, more

Neuroscience Research > 2011 > 71 > Supplement > e318

article

Syntheses of 2-NBDG analogues for monitoring stereoselective uptake of d-glucose

Toshihiro Yamamoto, Shin-ichi Tanaka, Sechiko Suga, Seiji Watanabe, more

Bioorganic & Medicinal Chemistry Letters > 2011 > 21 > 13 > 4088-4096

2-NBDG is a widely used fluorescent tracer for monitoring d-glucose uptake into single living cells. However, 2-NBDG alone is not sufficient for monitoring the net stereoselective uptake of d-glucose, unless its possible non-stereoselective uptake is properly evaluated. l-Glucose derivatives, which emit fluorescence distinct from that of 2-NBDG, should provide valuable information on the stereoselective...

article

Structure–activity relationship of marinostatin, a serine protease inhibitor isolated from a marine organism

Misako Taichi, Tohimasa Yamazaki, Kazuki Kawahara, Daisuke Motooka, more

Journal of Peptide Science > 16 > 7 > 329 - 336

A 12‐residue MST isolated from a marine organism is a potent serine protease inhibitor that has a double cyclic structure composed of two ester linkages formed between the β‐hydroxyl and β‐carboxyl groups, Thr³‐Asp⁹ and Ser⁸‐Asp¹¹. MST was synthesized by a regioselective esterification procedure employing two sets of orthogonally removable side‐chain protecting groups for the Asp and Ser/Thr residues...

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Evaluation of 2-NBDLG, the antipode of D-glucose tracer 2-NBDG, in SNr GABAergic neurons

Katsuhiro Nagatomo, Sechiko Suga, Seiji Watanabe, Ruka Echigo, more

Neuroscience Research > 2009 > 65 > Supplement 1 > S223

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Synthesis of 2-NBDLG, a fluorescent derivative of l-glucosamine; the antipode of d-glucose tracer 2-NBDG

Toshihiro Yamamoto, Yuji Nishiuchi, Tadashi Teshima, Hideaki Matsuoka, more

Tetrahedron Letters > 2008 > 49 > 48 > 6876-6878

2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-l-glucose [2-NBDLG] (2) is a long-awaited control substance compensating the non-specific uptake of 2-NBDG (1), which has been widely used as a fluorescent tracer for monitoring d-glucose uptake into single, living cells. A new synthetic method of optically pure l-glucosamine, which is not available as a natural product, has been developed. The...

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Optimization of the Oxidative Folding Reaction and Disulfide Structure Determination of Human α-Defensin 1, 2, 3 and 5

Shigeru Kubo, Kyoko Tanimura, Hideki Nishio, Naoyoshi Chino, more

International Journal of Peptide Research and Therapeutics > 2008 > 14 > 4 > 341-349

The optimal conditions were determined for oxidative folding of the reduced human α-defensins, HNP1, HNP2, HNP3 and HD5, preferentially into their native disulfide structures. Since the human α-defensin-molecule in both reduced and oxidized forms raised a solubility problem arising from its basic and hydrophobic compositions, buffer concentration had to be lowered and cosolvent, such as CH₃CN, had...

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Quantitative measurement of caspase-3 activity in a living starfish egg

Miki Sakaue, Yumiko Motoyama, Kayono Yamamoto, Tetsuo Shiba, more

Biochemical and Biophysical Research Communications > 2006 > 350 > 4 > 878-883

If not fertilized, synchronous apoptosis is induced in starfish eggs at ∼11h after stimulation with the hormone, 1-methyladenine. In this study, a membrane-impermeant substrate of caspase-3, acetyl-Asp-Glu-Val-Asp-coumarylamido-4-methanesulfonic acid (Ac-DEVD-CAMS), was synthesized and microinjected into a starfish egg. Caspase-3 activity in unfertilized egg was detected ∼30min before blebbing by...

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Site-Dependent Effect of O-Glycosylation on the Conformation and Biological Activity of Calcitonin ^†

Mizuka Tagashira, Hideki Iijima, Yukihiro Isogai, Masayuki Hori, more

Biochemistry > 2001 > 40 > 37 > 11090 - 11095

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Induction of Apoptotic Cell Death by Methylglyoxal and 3-Deoxyglucosone in Macrophage-Derived Cell Lines

Ayako Okado, Yoshimi Kawasaki, Yukiko Hasuike, Motoko Takahashi, more

Biochemical and Biophysical Research Communications > 1996 > 225 > 1 > 219-224

Production of 2-oxoaldehyde compounds increases during hyperglycemic conditions and is cytotoxic to susceptible cells. We have investigated the effects of methylglyoxal and 3-deoxyglucosone at physiological concentrations on monocytic leukemia U937 cells and other cell lines. Both ladder formation of DNA and nuclear fragmentation were observed in the cells treated with these agents, indicating that...

article

tRNA binding to programmed ribosomes increases the ribosomal affinity for tuberactinomycin O

Takeshi Yamada, Tadashi Teshima, Tetsuo Shiba, Knud H. Nierhaus

FEBS Letters > 1985 > 179 > 1 > 37-40

The binding of ¹ ⁴ C-labelled tuberactinomycin O was analysed in equilibrium dialysis cells. The ionic conditions and the concentration of the labelled drug used in the binding assays allowed the binding of just one drug molecule per non-programmed ribosome. Under these conditions, the occupation of the ribosomal P-site by deacylated tRNA ^P ^h ^e in the presence of poly(U)...

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