The kinetic characteristics of Rhizopus oryzae endo-polygalacturonase, RPG1, hydrolyzing galacturonic acid oligomers (GalpA)n were determined. RPG1 generates (GalpA)3 as a dominant product of polygalacturonic acid and (GalpA)4–6 hydrolysis. The enzyme can hydrolyze (GalpA)3, but hydrolysis occurs at a significantly lower rate relative to oligomers with a higher degree of polymerization. Hydrolysis...
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