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We developed a micro-PIXE system for the analysis of frozen cellular samples at atmospheric pressure under helium gas cooling. We analyzed bovine aortic endothelial (BAE) cells previously cultured in a medium containing bromodeoxyuridine (BrdU). It is known that these cells take BrdU into their nuclei at the S phase of their metabolic cycle. We examined the Br uptake for incubation times of 6, 12...
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