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The genotype of the recombinant yeast strain Y33 YF71-3 used in this study was, his, ade andsuc. Preliminary batch cultures in shake flask showed the expression level of atrial natriuretic peptide (ANP) was 1-2mg/L. In fed batch cultures carried out in a RIBE-5 fermenter, the cultures were fed with glucose and a mixture of YNB, Caa, adenine, histidine and leucine to improve cell growth and ANP...
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