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Herein, we report an electrochemical biosensor for the measurement of proteins and protease activity using carbon nanopowder paste electrode modified with tyrosinase. The measurement of proteins is based on the amperometric measurement of the enzymatic product, tyrosine, at low applied potential (i.e +0.05 V vs. Ag/AgCl), in a linear range from 1 μg/mL to 10 μg/mL for casein and albumin bovine serum...
A simple and sensitive amperometric biosensor was developed for the screening of potential xanthine oxidase inhibitors from medicinal plants. This biosensor was prepared by immobilization of xanthine oxidase on the surface of prussian blue modified screen-printed electrodes using nafion and glutaraldehyde.The developed biosensor showed a linear amperometric response at an applied potential of +0.05V...
A simple graphical method for the determination of reversible inhibition type, inhibition constant (Ki) and estimation of fifty percent of inhibition I50 of an enzyme reaction is described. The method consists of plotting experimental data as “degree of inhibition” versus the inhibitor concentration at two or more concentrations of substrate. Diagnosis of inhibition type is based on determination...
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