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The voltage‐gated proton channel, HV1, is crucial for innate immune responses. According to alternative hypotheses, protons either hop on top of an uninterrupted water wire or bypass titratable amino acids, interrupting the water wire halfway across the membrane. To distinguish between both hypotheses, the water mobility for the putative case of an uninterrupted wire is estimated. The predicted single‐channel...
Measurements of the unitary hydraulic conductivity of membrane channels, pf, may be hampered by difficulties in producing sufficient quantities of purified and reconstituted proteins. Low yield expression, the purely empiric choice of detergents, as well as protein aggregation and misfolding during reconstitution may result in an average of less than one reconstituted channel per large unilamellar...
Many membrane channels and transporters conduct water. Their reconstitution into giant unilamellar vesicles (GUVs) – here represented by the water tap – serves to assess their unitary hydraulic permeabilities. Boytsov, Hannesschlaeger, Horner, Siligan, and Pohl report the assay in article 1900450. It is based on measurements of (i) GUV volume changes by micropipette‐aspiration and (ii) pore density...
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