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NADPH‐dependent 5‐keto‐d‐gluconate reductase from Gluconobacter suboxydans IFO12528 (5KGR) catalyzes oxidoreduction between 5‐keto‐d‐gluconate and d‐gluconate with high specificity. 5KGR was expressed in Escherichia coli, purified and crystallized with 5‐keto‐d‐gluconate and NADPH using the sitting‐drop vapour‐diffusion method at 288 K. A crystal of the 5KGR–NADPH complex was obtained using reservoir...
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