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Osthole (1) as a natural product, has the potent anti-osteoporosis activity. In the present paper, a scaled-up biotransformation of 1 by Mucor spinosus AS 3.3450 was carried out for improving its activities and water-solubility. Nine metabolites were obtained in the biotransformation process of 1, and their structures were elucidated as 4′-hydroxyl-osthole (2), osthenol (3), osthenol-7-O-β-d-glucoside...
A eukaryotic expression system for secretory production of the protopectinase from Bacillus subtilis XZ2 in Pichia Pastoris was constructed in this paper. The protopectinase gene without native signal sequence was amplified by PCR and fused to the pPICZαA plasmid containing α-factor sequence encoding secretion signal from Saccharomyces cerevisiae. The heterologous gene was expressed under the AOX1...
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