Search results for: Nicholas M. Riley

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article

Sequencing Larger Intact Proteins (30-70 kDa) with Activated Ion Electron Transfer Dissociation

Nicholas M. Riley, Michael S. Westphall, Joshua J. Coon

Journal of The American Society for Mass Spectrometry > 2018 > 29 > 1 > 140-149

The analysis of intact proteins via mass spectrometry can offer several benefits to proteome characterization, although the majority of top-down experiments focus on proteoforms in a relatively low mass range (<30 kDa). Recent studies have focused on improving the analysis of larger intact proteins (up to ~75 kDa), but they have also highlighted several challenges to be addressed. One major hurdle...

article

Negative Electron Transfer Dissociation Sequencing of Increasingly Sulfated Glycosaminoglycan Oligosaccharides on an Orbitrap Mass Spectrometer

Franklin E. Leach, Nicholas M. Riley, Michael S. Westphall, Joshua J. Coon, more

Journal of The American Society for Mass Spectrometry > 2017 > 28 > 9 > 1844-1854

The structural characterization of sulfated glycosaminoglycan (GAG) carbohydrates remains an important target for analytical chemists attributable to challenges introduced by the natural complexity of these mixtures and the defined need for molecular-level details to elucidate biological structure–function relationships. Tandem mass spectrometry has proven to be the most powerful technique for this...

article

Sulfur Pentafluoride is a Preferred Reagent Cation for Negative Electron Transfer Dissociation

Matthew J. P. Rush, Nicholas M. Riley, Michael S. Westphall, John E. P. Syka, more

Journal of The American Society for Mass Spectrometry > 2017 > 28 > 7 > 1324-1332

Negative mode proteome analysis offers access to unique portions of the proteome and several acidic post-translational modifications; however, traditional collision-based fragmentation methods fail to reliably provide sequence information for peptide anions. Negative electron transfer dissociation (NETD), on the other hand, can sequence precursor anions in a high-throughput manner. Similar to other...

article

Proteomics Moves into the Fast Lane

Nicholas M. Riley, Alexander S. Hebert, Joshua J. Coon

Cell Systems > 2016 > 2 > 3 > 142-143

Three studies demonstrate the potential of state-of-the-art mass spectrometry-based proteomics for rapid, deep characterization of proteomes.

article

Enhanced Dissociation of Intact Proteins with High Capacity Electron Transfer Dissociation

Nicholas M. Riley, Christopher Mullen, Chad R. Weisbrod, Seema Sharma, more

Journal of The American Society for Mass Spectrometry > 2016 > 27 > 3 > 520-531

Electron transfer dissociation (ETD) is a valuable tool for protein sequence analysis, especially for the fragmentation of intact proteins. However, low product ion signal-to-noise often requires some degree of signal averaging to achieve high quality MS/MS spectra of intact proteins. Here we describe a new implementation of ETD on the newest generation of quadrupole-Orbitrap-linear ion trap Tribrid,...

article

A Calibration Routine for Efficient ETD in Large-Scale Proteomics

Christopher M. Rose, Matthew J. P. Rush, Nicholas M. Riley, Anna E. Merrill, more

Journal of The American Society for Mass Spectrometry > 2015 > 26 > 11 > 1848-1857

Electron transfer dissociation (ETD) has been broadly adopted and is now available on a variety of commercial mass spectrometers. Unlike collisional activation techniques, optimal performance of ETD requires considerable user knowledge and input. ETD reaction duration is one key parameter that can greatly influence spectral quality and overall experiment outcome. We describe a calibration routine...

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ELECTRON TRANSFER DISSOCIATION (3)
INTACT PROTEINS (2)
MASS SPECTROMETRY (2)
ORBITRAP (2)
PROTEOMICS (2)
CALIBRATION (1)
ETD (1)
GLYCOSAMINOGLYCAN (1)
INSTRUMENTATION (1)
ION/ION (1)
ION/ION REACTIONS (1)
ION–ION REACTIONS (1)
KINETICS (1)
NEGATIVE ELECTRON TRANSFER DISSOCIATION (1)
NEGATIVE MODE (1)
NETD (1)
PEPTIDE ANIONS (1)
PHOTO-ACTIVATION (1)
SULFATED CARBOHYDRATE (1)
TANDEM MASS SPECTROMETRY (1)
TOP-DOWN PROTEOMICS (1)
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Springer (5)
Elsevier (1)

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Search results for: Nicholas M. Riley

Sequencing Larger Intact Proteins (30-70 kDa) with Activated Ion Electron Transfer Dissociation

Negative Electron Transfer Dissociation Sequencing of Increasingly Sulfated Glycosaminoglycan Oligosaccharides on an Orbitrap Mass Spectrometer

Sulfur Pentafluoride is a Preferred Reagent Cation for Negative Electron Transfer Dissociation

Proteomics Moves into the Fast Lane

Enhanced Dissociation of Intact Proteins with High Capacity Electron Transfer Dissociation

A Calibration Routine for Efficient ETD in Large-Scale Proteomics

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