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A DNA segment encoding a novel α‐amylase was cloned from the Antarctic psychrotolerant fungi Geomyces pannorum. The gene has an open reading frame of 1761 bp, deduced 586 amino acids, revealing a relatively low homology to α‐amylases from diverse fungal genera. The α‐amylase gene was overexpressed in Pichia pastoris, and the recombinant α‐amylase was purified and characterized. Enzymatic activity...
Filamentous fungi have increasingly been used as hosts for heterologous protein production because of their high secretion capability and ability to add eukaryotic post-translational modifications. In this study, a novel uracil-deficient Aspergillus transformation system, which was based on an orotate phosphoribosyltransferase (pyrF) nutritional selection marker, was discovered. Additionally, a universal,...
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