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The inhibitory potential of novel anti-platelet aggregatory cilostamide analogues on phosphodiesterase (PDE) isozyme activities was investigated with recombinant PDE isozymes expressed in a baculovirus/Sf9 expression system. The recombinant enzymes (PDE1-PDE5 and PDE7) showed K m values and sensitivities to selective inhibitors similar to those reported previously for native enzymes purified...
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