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A label‐free mass spectrometric strategy was used to examine the effect of 5‐fluorouracil (5‐FU) on the primary and metastatic colon carcinoma cell lines, SW480 and SW620, with and without treatment. 5‐FU is the most common chemotherapeutic treatment for colon cancer. Pooled biological replicates were analyzed by nanoLC‐MS/MS and protein quantification was determined via spectral counting. Phenotypic...
Background.Prior studies have suggested the multifactorial nature of mitomycin C (MMC) resistance. However, the relative importance of the different resistance mechanisms is unknown.Materials and methods.A panel of colon cancer cell lines with levels of MMC resistance from 2- to 15-fold compared to the parent line HT-29 was produced by repeated MMC exposure. Cell survival was measured using clonogenic...
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