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To understand the molecular mechanism of gibberellin-dependent gene regulation, the effect of three phosphatase inhibitors on the germination of rice seeds and the expression of a target gene, the α-amylase gene, Osamy-c, were measured. We found that okadaic acid, microcystin-LR, and calyculin A, which are known to specifically inhibit Ser/Thr phosphatases 1 and 2A, strongly inhibit the expression...
A Δ 1 -pyrroline-5-carboxylate synthetase (P5CS) cDNA from mothbean (Vigna aconitifolia L.) was introduced into rice (Oryza sativa L.) genome by the biolistic method. Expression of this P5CS transgene under the control of a stress-inducible promoter led to stress-induced overproduction of the P5CS enzyme and proline accumulation in transgenic rice plants. Second-generation (R 1 ) transgenic...
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