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Bulked segregant analysis (BSA) using the technique of amplified fragment length polymorphism (AFLPTM) was carried out on backcross hybrids between the important pasture species white clover and its relative Trifolium ambiguum. The introgression of the rhizomatous trait fromTrifolium ambiguum, previously shown to be associated with enhanced drought tolerance but which takes eighteen months to manifest...
The nitrogen relations of an inbred line of white clover (Trifolium repens L.) thought to exhibit an abnormally low capacity for NO3− uptake (line LNU) were compared with a line regarded as normal with respect to NO3− uptake (line NNU). Growth, nodulation, N2 fixation and NO3− uptake were measured over 7 weeks in flowing solution culture (Experiment 1) by plants dependent for N acquisition on either...
Interspecific hybridisation with Trifolium nigrescens Viv. is a possible strategy to improve the reproductive potential of white clover (Trifolium repens L.). Following the development of a fertile F1 hybrid, three generations of backcrossing have been carried out usingT. repens as the recurrent parent. Vegetative characteristics, stolon growth and seed yield components of the backcross (BC) 2 and...
Two generations of novel backcross hybrids between white clover (Trifolium repens L.) and the related species Trifolium ambiguum,with white clover as the recurrent parent, were analysed for forage quality characteristics. Water soluble carbohydrate (WSC) levels, nitrogen concentrations (%N), and dry matter digestibility (DMD) were recorded for the hybrids and parental species grown in deep soil bins...
Interspecific hybridisation with the close relative, Trifolium nigrescens (Ball clover) is a possible strategy to achieve increased reproductive potential of white clover (Trifolium repens). Fertile F1 plants have been used as the basis for two generations of backcrossing to T. repens as the recurrent parent. F1 and backcrossed plants were assessed in both glasshouse and field for a range of morphological...
The effects of two generations of divergent selection for stolon diameter and stolon amount on the pattern of phenotypic correlations between important agronomic traits in white clover (Trifolium repens L.) were studied. The effects of selection were: the proportions of the variances of traits under direct selection were considerably lower within selection lines than overall and this was associated...
Growth, dry matter partitioning between shoots and roots, and extent of nodulation were characterised in four novel self-fertile highly inbred lines (referred to as A, B, C and D) of white clover (Trifolium repens L.) thought to differ fundamentally in their nitrogen relations and therefore regarded as candidate material for genetic mapping. Plants were inoculated with a mixture of three strains of...
N2 fixation, nitrate uptake, and tissue N levels werecompared in four novel self-fertile inbred lines (referred to as A, B, C andD) of white clover (Trifolium repens L.) believed to differ markedly intheir nitrogen relations. Plants were inoculated with a mixture of Rhizobiumstrains and grown in flowing nutrient solutions without mineral N for 18 dprior to supplying half the plants with 20 µM $$NO_3^...
Inbred lines of white clover (Trifolium repens L.) have been produced utilising the rare self-fertility (Sf) allele. Twenty-two lines of four distinct groups have been maintained through five generations of selfing by single seed descent. Fourteen lines were used in crosses to produce F1s. Both parents and hybrids were analysed for a range of morphological characters. Positive heterosis for dry matter...
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