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In this work we have shown for the first time the effect of Met65Thr substitution on the activity of catechol 2,3-dioxygenase (C23O) from Planococcus sp. strain S5. Even though the 3D model does not reveal the influence of an amino acid substitution on the enzyme active site structure, the Thr65 variant differed in lower pH optimum and lower Km from the wild type enzyme. Activity measurements with...
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