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We demonstrate three-photon fluorescence adaptive-optics system based on 1660-nm femtosecond pulses and MEMS spatial light modulator. We use the higher nonlinearity of the signal resulting in ×700 improvement in fluorescence beads signal after aberrations correction.
We demonstrate tunable soliton generation in excess of 3 MW peak power in the 1,700 nm spectral region using a solid-core photonic crystal rod pumped by a compact femtosecond fiber source. This system can potentially be used for in vivo deep tissue multiphoton microscopy.
We characterize the diagnostic performance of a multiphoton GRIN endoscope using human prostate samples obtained from radical prostatectomy surgery. Ex vivo images of benign and tumor areas and images of peri-prostatic tissue are shown.
We demonstrate three-photon microscopy (3PM) at 1300-nm excitation for imaging neuronal activity as deep as layer-6 (L6) of adult mouse cortex using genetically-encoded calcium indicators. In vivo imaging of subcortical, GFP-labeled neurons is also demonstrated.
We present a dual modality microendoscope with a miniature, 9× optical zoom lens that is capable of large field-of-view (1.3 mm) reflectance imaging and high-resolution (0.57 mm) multiphoton fluorescence imaging of unstained tissues.
Self-starting of mode-locking in normal-dispersion Yb fiber lasers is studied experimentally and theoretically. Starting can be initiated by quantum noise or relaxation oscillations, and is much faster than in soliton lasers.
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