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Crude extracts of Ataulfo exhibited polyphenol oxidase (PPO) activity with pyrogallol, 3-methylcatechol, catechol, gallic acid, and protocatechuic acid. The substrate dependent pH optima ranged from pH 5.4 to 6.4 with Michaelis–Menten constants between 0.84±0.09 and 4.6±0.7mM measured in MES or phosphate buffers. The use of acetate buffers resulted in larger Michaelis–Menten constants, up to 14.62±2...