Search results for: Juan D. Chavez

Items from 1 to 12 out of 12 results

article

Applications and advancements of FT‐ICR‐MS for interactome studies

Juan D. Chavez, Sung‐Gun Park, Jared P. Mohr, James E. Bruce

Mass Spectrometry Reviews > 41 > 2 > 248 - 261

The set of all intra‐ and intermolecular interactions, collectively known as the interactome, is currently an unmet challenge for any analytical method, but if measured, could provide unparalleled insight on molecular function in living systems. Developments and applications of chemical cross‐linking and high‐performance mass spectrometry technologies are beginning to reveal details on how proteins...

article

Characterizing environmental stress responses of aposymbiotic Astrangia poculata to divergent thermal challenges

Daniel M. Wuitchik, Adeline Almanzar, Brooke E. Benson, Sara Brennan, more

Molecular Ecology > 30 > 20 > 5064 - 5079

Anthropogenic climate change threatens corals globally and both high and low temperatures are known to induce coral bleaching. However, coral stress responses across wide thermal breadths remain understudied. Disentangling the role of symbiosis on the stress response in obligately symbiotic corals is challenging because this response is inherently coupled with nutritional stress. Here, we leverage...

article

Crosslinking mass spectrometry: A link between structural biology and systems biology

Xiaoting Tang, Helisa H. Wippel, Juan D. Chavez, James E. Bruce

Protein Science > 30 > 4 > 773 - 784

Protein structure underpins functional roles in all biological processes; therefore, improved understanding of protein structures is of fundamental importance in nearly all biological and biomedical research areas. Traditional techniques such as X‐ray crystallography and more recently, cryo‐EM, can reveal structural features on isolated proteins/protein complexes at atomic resolution level and have...

article

Insights in luteovirid structural biology guided by chemical cross-linking and high resolution mass spectrometry

Mariko M. Alexander, Jared P. Mohr, Stacy L. DeBlasio, Juan D. Chavez, more

Virus Research > 2017 > 241 > C > 42-52

Interactions among plant pathogenic viruses in the family Luteoviridae and their plant hosts and insect vectors are governed by the topology of the viral capsid, which is the sole vehicle for long distance movement of the viral genome. Previous application of a mass spectrometry-compatible cross-linker to preparations of the luteovirid Potato leafroll virus (PLRV; Luteoviridae: Polerovirus) revealed...

article

In Vivo Conformational Dynamics of Hsp90 and Its Interactors

Juan D. Chavez, Devin K. Schweppe, Jimmy K. Eng, James E. Bruce

Cell Chemical Biology > 2016 > 23 > 6 > 716-726

Hsp90 belongs to a family of some of the most highly expressed heat shock proteins that function as molecular chaperones to protect the proteome not only from the heat shock but also from other misfolding events. As many client proteins of Hsp90 are involved in oncogenesis, this chaperone has been the focus of intense research efforts. Yet, we lack structural information for how Hsp90 interacts with...

article

Host-Microbe Protein Interactions during Bacterial Infection

Devin K. Schweppe, Christopher Harding, Juan D. Chavez, Xia Wu, more

Chemistry & Biology > 2015 > 22 > 11 > 1521-1530

Interspecies protein-protein interactions are essential mediators of infection. While bacterial proteins required for host cell invasion and infection can be identified through bacterial mutant library screens, information about host target proteins and interspecies complex structures has been more difficult to acquire. Using an unbiased chemical crosslinking/mass spectrometry approach, we identified...

article

Probing the Protein Interaction Network of Pseudomonas aeruginosa Cells by Chemical Cross-Linking Mass Spectrometry

Arti T. Navare, Juan D. Chavez, Chunxiang Zheng, Chad R. Weisbrod, more

Structure > 2015 > 23 > 4 > 762-773

In pathogenic Gram-negative bacteria, interactions among membrane proteins are key mediators of host cell attachment, invasion, pathogenesis, and antibiotic resistance. Membrane protein interactions are highly dependent upon local properties and environment, warranting direct measurements on native protein complex structures as they exist in cells. Here we apply in vivo chemical cross-linking mass...

article

SILACtor: Software To Enable Dynamic SILAC Studies

Michael R. Hoopmann, Juan D. Chavez, James E. Bruce

Analytical Chemistry > 2011 > 83 > 22 > 8403 - 8410

article

Site-specific proteomic analysis of lipoxidation adducts in cardiac mitochondria reveals chemical diversity of 2-alkenal adduction

Juan D. Chavez, Jianyong Wu, William Bisson, Claudia S. Maier

Journal of Proteomics > 2011 > 74 > 11 > 2417-2429

The modification of proteins by lipid peroxidation products has been linked to numerous diseases and age-related disorders. Here we report on the identification of endogenous protein targets of electrophilic 2-alkenals in cardiac mitochondria. An aldehyde/keto-specific chemical labeling and affinity strategy in combination with LC–MS/MS resulted in 39 unique lipoxidation sites on 27 proteins. Several...

article

Quantification of Protein−Protein Interactions with Chemical Cross-Linking and Mass Spectrometry

Juan D. Chavez, Neal L. Liu, James E. Bruce

Journal of Proteome Research > 2011 > 10 > 4 > 1528 - 1537

article

A targeted mass spectrometry-based approach for the identification and characterization of proteins containing α-aminoadipic and γ-glutamic semialdehyde residues

Juan D. Chavez, William H. Bisson, Claudia S. Maier

Analytical and Bioanalytical Chemistry > 2010 > 398 > 7-8 > 2905-2914

The site-specific identification of α-aminoadipic semialdehyde (AAS) and γ-glutamic semialdehyde (GGS) residues in proteins is reported. Semialdehydic protein modifications result from the metal-catalyzed oxidation of Lys or Arg and Pro residues, respectively. Most of the analytical methods for the analysis of protein carbonylation measure change to the global level of carbonylation and fail to provide...

article

Supercomplexes of the mitochondrial electron transport chain decline in the aging rat heart

Luis A. Gómez, Jeffrey S. Monette, Juan D. Chavez, Claudia S. Maier, more

Archives of Biochemistry and Biophysics > 2009 > 490 > 1 > 30-35

Accumulation of mitochondrial electron transport chain (ETC) defects is a recognized hallmark of the age-associated decline in cardiac bioenergetics; however, the molecular events involved are only poorly understood. In the present work, we hypothesized that age-related ETC deterioration stemmed partly from disassociation of large solid-state macromolecular assemblies termed “supercomplexes”. Mitochondrial...

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