Search results for: Sindhu Mathew

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Xylo- and arabinoxylooligosaccharides from wheat bran by endoxylanases, utilisation by probiotic bacteria, and structural studies of the enzymes

Sindhu Mathew, Anna Aronsson, Eva Nordberg Karlsson, Patrick Adlercreutz

Applied Microbiology and Biotechnology > 2018 > 102 > 7 > 3105-3120

Xylooligosaccharides (XOS) and arabinoxylooligosaccharides (AXOS) were produced from the insoluble arabinoxylan fraction of pretreated wheat bran by endoxylanases. The glycoside hydrolase (GH) family 10 xylanases GsXyn10A from Geobacillus stearothermophilus and RmXyn10A-CM from Rhodothermus marinus produced the AXOS A³X, A²XX and A^2 + 3XX in addition to XOS. RmXyn10A-CM also produced XA^2 + 3XX due...

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Extraction of soluble arabinoxylan from enzymatically pretreated wheat bran and production of short xylo-oligosaccharides and arabinoxylo-oligosaccharides from arabinoxylan by glycoside hydrolase family 10 and 11 endoxylanases

Sindhu Mathew, Eva Nordberg Karlsson, Patrick Adlercreutz

Journal of Biotechnology > 2017 > 260 > C > 53-61

The enzymatic, ecofriendly pretreatment of wheat bran with α-amylase from Bacillus amyloliquifaciens or B. licheniformis at 90°C for 1.5h followed by Neutrase at 50°C for 4h, aqueous liquefaction at 121°C for 15h and ethanol precipitation enabled the production of soluble arabinoxylan (AX) with purity of 70.9% and 68.4% (w/w) respectively. Process alternatives tried, to simplify the process and curtail...

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Antioxidant property and chemical profile of pyroligneous acid from pineapple plant waste biomass

Sindhu Mathew, Zainul Akmar Zakaria, Nur Fashya Musa

Process Biochemistry > 2015 > 50 > 11 > 1985-1992

Pyroligneous acid was produced by subjecting the stem and leaves of pineapple plants from a local plantation area to slow pyrolysis in a charcoal kiln for 48h. The concentrated pyroligneous acid from pineapple plant waste biomass (CPAP: Concentrated pyroligneous acid from pineapple waste biomass) and its dichloromethane extract (DCPAP: Dichloromethane extract of concentrated pyroligneous acid from...

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Reactivity of phenolic compounds towards free radicals under in vitro conditions

Sindhu Mathew, T. Emilia Abraham, Zainul Akmar Zakaria

Journal of Food Science and Technology > 2015 > 52 > 9 > 5790-5798

The free radical scavenging activity and reducing power of 16 phenolic compounds including four hydroxycinnamic acid derivatives namely ferulic acid, caffeic acid, sinapic acid and p-coumaric acid, benzoic acid and its derivatives namely protocatechuic acid, gallic acid and vanillic acid, benzene derivatives namely vanillin, vanillyl alcohol, veratryl alcohol, veratraldehyde, pyrogallol, guaiacol...

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Pyroligneous acid—the smoky acidic liquid from plant biomass

Sindhu Mathew, Zainul Akmar Zakaria

Applied Microbiology and Biotechnology > 2015 > 99 > 2 > 611-622

Pyroligneous acid (PA) is a complex highly oxygenated aqueous liquid fraction obtained by the condensation of pyrolysis vapors, which result from the thermochemical breakdown or pyrolysis of plant biomass components such as cellulose, hemicellulose, and lignin. PA produced by the slow pyrolysis of plant biomass is a yellowish brown or dark brown liquid with acidic pH and usually comprises a complex...

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Regioselective glycosylation of hydroquinone to α-arbutin by cyclodextrin glucanotransferase from Thermoanaerobacter sp.

Sindhu Mathew, Patrick Adlercreutz

Biochemical Engineering Journal > 2013 > 79 > Complete > 187-193

Hydroquinone glycosides were produced by transglycosylation reactions catalyzed by cyclodextrin glucanotransferase (CGTase) from Thermoanaerobacter sp. (Toruzyme ^® 3.0L). The reactions were carried out in an aqueous system containing hydroquinone (HQ) and maltodextrin as acceptor and donor substrate molecules respectively. The conditions for the synthesis of hydroquinone glucoside (α-arbutin)...

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Enzymatic synthesis of piceid glycosides by cyclodextrin glucanotransferase

Sindhu Mathew, Martin Hedström, Patrick Adlercreutz

Process Biochemistry > 2012 > 47 > 3 > 528-532

Novel glycosides of piceid (3,4′-5-trihydroxy stilbene 3-O-β-d-glucoside) were produced by the transglycosylation reactions of cyclodextrin glucanotransferase (CGTase) from Bacillus macerans, with piceid (PicG ₁ ) and maltodextrin as the acceptor and donor substrates, respectively. The reactions were performed at 40°C with 2.56mM piceid (0.1% w/v) and maltodextrin (5% w/v) in 0.02M citrate...

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Cyclodextrin glucanotransferase (CGTase) catalyzed synthesis of dodecyl glucooligosides by transglycosylation using α-cyclodextrin or starch

Sindhu Mathew, Patrick Adlercreutz

Carbohydrate Polymers > 2012 > 87 > 1 > 574-580

Dodecyl glucooligosides, a class of interesting non ionic surfactant molecules were synthesized by cyclodextrin glucanotransferase from Bacillus macerans using either α-cyclodextrin (α-CD) or soluble starch as glycosyl donor and dodecyl β-d-glucoside (C ₁₂ G ₁ ) or dodecyl β-d-maltoside (C ₁₂ G ₂ ) as acceptor substrates. The primary coupling products obtained in the...

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Analysis of carbonyl compounds in sea buckthorn for the evaluation of triglyceride oxidation, by enzymatic hydrolysis and derivatisation methodology

Sindhu Mathew, Carl Grey, Kimmo Rumpunen, Patrick Adlercreutz

Food Chemistry > 2011 > 126 > 3 > 1399-1405

Carbonyl compounds formed in sea buckthorn berry (Hippophae rhamnoides) and oil samples as a result of lipid oxidation were determined by enzymatic hydrolysis followed by derivatisation with 2,4-dinitrophenylhydrazine and analysed by LC–UV and electrospray ionisation (ESI) tandem mass spectrometry (MS/MS). Several classes of carbonyl compounds such as saturated, unsaturated, linear and branched aldehydes...

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Mediator facilitated, laccase catalysed oxidation of granular potato starch and the physico-chemical characterisation of the oxidized products

Sindhu Mathew, Patrick Adlercreutz

Bioresource Technology > 2009 > 100 > 14 > 3576-3584

The primary hydroxyl groups in potato starch were selectively oxidized to the corresponding aldehyde and carboxylic acid functionalities by mediators like TEMPO, using laccase from fungi as catalytic oxidant and oxygen as the primary oxidant. Oxidized starch products with degree of substitution (DS _CHO ranging from 0.16 to 16.4/100AGU and DS _COOH from 0.01 to 3.71 carboxyl groups/100AGU)...

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Characterisation of ferulic acid incorporated starch–chitosan blend films

Sindhu Mathew, T. Emilia Abraham

Food Hydrocolloids > 2008 > 22 > 5 > 826-835

The present study has evaluated the characteristics of ferulic acid incorporated starch–chitosan blend films, which finds possible application as an edible film or coating. The barrier properties of the blend films were found to improve considerably upon the incorporation of oxidized ferulic acid. Studies on the surface morphology of the control blend films by light microscopy, SEM and AFM revealed...

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Rapid conversion of ferulic acid to 4-vinyl guaiacol and vanillin metabolites by Debaryomyces hansenii

Sindhu Mathew, T. Emilia Abraham, S. Sudheesh

Journal of Molecular Catalysis. B, Enzymatic > 2007 > 44 > 2 > 48-52

Debaryomyces hansenii, an isolated yeast strain metabolized ferulic acid to an intermediate compound, namely 4-vinyl guaiacol, by the non oxidative decarboxylation of its side chain. This bioconversion is a highly value added process as 4-vinyl guaiacol is nearly 40 times costlier than ferulic acid. The major degradation products were isolated and identified by thin layer chromatography, high performance...

article

Physico-chemical characterization of starch ferulates of different degrees of substitution

Sindhu Mathew, T. Emilia Abraham

Food Chemistry > 2007 > 105 > 2 > 579-589

Starch ferulates were prepared by reacting potato starch with ferulic acid chloride, using pyridine as a catalyst in dimethyl sulfoxide. Starch ferulates of different degrees of substitution (DS) were prepared and their formation was confirmed by the presence of the carbonyl signal around 1726cm ⁻¹ in the FT-IR spectra. The thermal characteristics of the native starch and starch ferulates...

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Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models

Sindhu Mathew, T. Emilia Abraham

Food Chemistry > 2006 > 94 > 4 > 520-528

The antioxidant activities of the methanolic extract of Cinnamomum verum barks (CBE) were evaluated with reference to antioxidant compounds like butylated hydroxyl anisole, trolox and ascorbic acid. By virtue of their hydrogen donating ability, all of the tested compounds and CBE exhibited reducing power. They were found to be potent in free radical scavenging activity especially against DPPH radicals...

article

In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies

Sindhu Mathew, T. Emilia Abraham

Food and Chemical Toxicology > 2006 > 44 > 2 > 198-206

The free radical scavenging capacity and antioxidant activities of the methanolic extract of Cinnamomum verum leaf (CLE) were studied and compared to antioxidant compounds like trolox, butylated hydroxyl anisole, gallic acid and ascorbic acid. The CLE exhibited free radical scavenging activity, especially against DPPH radical and ABTS radical cation. They also exhibited reducing power and metal ion...

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Studies on the production of feruloyl esterase from cereal brans and sugar cane bagasse by microbial fermentation

Sindhu Mathew, T. Emilia Abraham

Enzyme and Microbial Technology > 2005 > 36 > 4 > 565-570

Production of ferulic acid, a precursor of vanillin from cereal bran is an attractive option due to the high price and demand for natural vanillin in the international arena and feruloyl esterase has been identified as the key enzyme involved. The methyl ester of ferulic acid was used as substrate for assaying the enzyme activity. Among the different strains of fungi screened on pretreated wheat bran...

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