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A simple procedure for the isolation of profilin mutants having a reduced capacity to bind poly(l-proline) is used to isolate two mutants of human profilin I, W3N and H133S. Binding of the mutants to poly(l-proline), actin, and phosphatidylinositol (4,5)-bisphosphate (PIP2) was studied. Both mutations abolished the poly(l-proline)-binding activity of profilin. This suggests that the arrangement of...
We are investigating structure–function relationships in profilin and actin by site-specific mutagenesis using a yeast,Saccharomyces cerevisiae,expression system to produce wild-type and mutant proteins. This paper shows that deleting proline 96 and threonine 97, which are located close to the major actin binding site on profilin, did not significantly alter the interaction between profilin and phosphatidylinositol...
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