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DifferentiatingDictyostelium discoideumamoebae respond chemotactically towards the attractant CAMP. To test whether chemotaxis requires the establishment of a spatial gradient of the cytosolic calcium concentration ([Ca 2+ ] i ) we scrape-loaded calcium chelating agents with different affinities for Ca 2+ into the cytosol of the cells. The buffers were 1,2-bis(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic...