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The mechanism by which ATP primes for subsequent macrophage-derived chemokine (MDC) mediated intracellular calcium (Ca2+i) responses at the human CCR4 receptor stably expressed in Chinese hamster ovary (CHO) cells was investigated. MDC alone was unable to elicit a Ca2+i response, but pre-stimulation of cells with ATP enabled a subsequent MDC-mediated Ca2+i response with a pEC50 of 8.660.16. The maximal...
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