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Protein self-assemblies modulate protein activities over biological timescales that can exceed the lifetimes of the proteins or even the cells that harbor them. We hypothesized that these timescales relate to kinetic barriers inherent to the nucleation of ordered phases. To investigate nucleation barriers in living cells, we developed distributed amphifluoric FRET (DAmFRET). DAmFRET exploits a photoconvertible...
A J integer and CTOD test are usually done on materials undergoes plastic deformation prior to failure. The testing material more or less resembles the original one, although dimensions can be reduced proportionally. More than 3 tests are done to minimize any experimental deviations. The specimen is placed on the work table and a notch is created exactly at the centre. The crack should be generated...
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