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This paper presents a cellular force measurement technique that allows for mechanical characterization of mouse oocytes during microinjection (i.e., in situ) without requiring a separate characterization process. The technique employs an elastic cell holding device and a sub-pixel computer vision tracking algorithm to resolve cellular forces in real time with a nanonewton force measurement resolution...
This paper presents the use of a monolithic, force-feedback MEMS (microelectomechanical systems) microgripper for characterizing both elastic and viscoelastic properties of highly deformable hydrogel microcapsules (15-25 mum) at wet state during micromanipulation. The single-chip microgripper integrates an electrothermal microactuator and two capacitive force sensors, one for contact detection (force...
This paper presents an automated cell contour visual measurement technique and a data synchronization mechanism for real-time, high-accuracy mechanical characterization of individual cells with micropipette aspiration. A computer vision tracking algorithm is developed for automatically measuring cell deformation parameters in real time (30 Hz) with a resolution down to 0.21 pixel, significantly enhancing...
Manipulation and characterization of individual biological cells require cellular forces be precisely measured in real time. This paper presents a computer vision-based cellular force measurement platform that allows for the use a single vision sensor to simultaneously obtain two forms of feedback (i.e., vision and force). A novel silicone elastomer-based cell holding device and a sub-pixel visual...
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