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Human kidney 293 TSA cells were transfected by a calcium phosphate method with human formylpeptide and C5a receptor cDNAs with high efficiency. Formylpeptide receptor positive transfectants expressed a total of 968,000 +/- 34,000 receptors per cell with two affinity states (K d s of ca. 0.43 nM and 39 nM), which in the presence of 100μM GTPγS decreased by ca. 4-fold the number of high-affinity...